Hatakeyama S, Ogasawara K, Fukushi N, Iwabuchi C, Iwabuchi K, Wang B Y, Kajiwara M, Good R A, Onoé K
Section of Pathology, Hokkaido University, Sapporo, Japan.
Acta Pathol Jpn. 1990 Jun;40(6):391-401. doi: 10.1111/j.1440-1827.1990.tb01578.x.
Lethally irradiated SJL/J mice were reconstituted with B10 bone marrow cells, and the process of thymic reconstitution by donor-derived cells positive for I-A or V beta 8 molecules was investigated. The donor-derived Ia+ cells appeared in the medulla on day 7 after reconstitution. The Ia+ cells became confluent up to day 14, and the cellularity in the medulla on day 17 was almost the same as that in the normal thymus. Dull V beta 8+ thymocytes were first recognized in the cortex on day 10 and were identifiable in the medulla by day 14. The V beta 8+ cells seemed to be mainly CD4+8+ double-positive. Furthermore, most of the V beta 8+ cells in the medulla of chimeras given cyclosporin A for 3 weeks after reconstitution appeared to be CD4+8+ thymocytes which bear a low concentration of TCR exist in the thymic medulla at a relatively early stage when donor-derived Ia+ cells have already settled there. The coincidental appearance and coexistence of Ia+ cells and TCR+ thymocytes in the medulla suggest that these histological characteristics may be related to the selection of thymocytes in this area.
用B10骨髓细胞重建受到致死剂量照射的SJL/J小鼠，研究供体来源的I-A或Vβ8分子阳性细胞进行胸腺重建的过程。重建后第7天，供体来源的Ia+细胞出现在髓质中。到第14天，Ia+细胞融合成片，第17天髓质中的细胞数量与正常胸腺中的几乎相同。迟钝的Vβ8+胸腺细胞在第10天首次在皮质中被识别，到第14天在髓质中也可识别。Vβ8+细胞似乎主要是CD4+8+双阳性细胞。此外，重建后给予环孢素A 3周的嵌合体小鼠，其髓质中大多数Vβ8+细胞似乎是CD4+8+胸腺细胞，当供体来源的Ia+细胞已经在胸腺髓质中定居时，这些细胞在相对较早阶段就存在于胸腺髓质中，且其TCR浓度较低。Ia+细胞和TCR+胸腺细胞在髓质中的同时出现和共存表明，这些组织学特征可能与该区域胸腺细胞的选择有关。
