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粘质沙雷氏菌对二乙酮生物降解的动力学研究。

Kinetics of biodegradation of diethylketone by Arthrobacter viscosus.

机构信息

IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal.

出版信息

Biodegradation. 2012 Feb;23(1):81-92. doi: 10.1007/s10532-011-9488-7. Epub 2011 Jun 17.

Abstract

The performance of an Arthrobacter viscosus culture to remove diethylketone from aqueous solutions was evaluated. The effect of initial concentration of diethylketone on the growth of the bacteria was evaluated for the range of concentration between 0 and 4.8 g/l, aiming to evaluate a possible toxicological effect. The maximum specific growth rate achieved is 0.221 h(-1) at 1.6 g/l of initial diethylketone concentration, suggesting that for higher concentrations an inhibitory effect on the growth occurs. The removal percentages obtained were approximately 88%, for all the initial concentrations tested. The kinetic parameters were estimated using four growth kinetic models for biodegradation of organic compounds available in the literature. The experimental data found is well fitted by the Haldane model (R (2) = 1) as compared to Monod model (R (2) = 0.99), Powell (R (2) = 0.82) and Loung model (R (2) = 0.95). The biodegradation of diethylketone using concentrated biomass was studied for an initial diethylketone concentration ranging from 0.8-3.9 g/l in a batch with recirculation mode of operation. The biodegradation rate found followed the pseudo-second order kinetics and the resulting kinetic parameters are reported. The removal percentages obtained were approximately 100%, for all the initial concentrations tested, suggesting that the increment on the biomass concentration allows better results in terms of removal of diethylketone. This study showed that these bacteria are very effective for the removal of diethylketone from aqueous solutions.

摘要

评估了粘质沙雷氏菌培养物去除水溶液中二乙酮的性能。评估了初始二乙酮浓度在 0 到 4.8 g/l 范围内对细菌生长的影响,目的是评估可能的毒理学效应。在 1.6 g/l 的初始二乙酮浓度下,最大比生长速率达到 0.221 h(-1),表明对于更高的浓度,生长会受到抑制。在所有测试的初始浓度下,去除率约为 88%。使用文献中可用的四种用于有机化合物生物降解的生长动力学模型来估计动力学参数。与 Monod 模型 (R (2) = 0.99)、Powell 模型 (R (2) = 0.82) 和 Loung 模型 (R (2) = 0.95) 相比,实验数据很好地符合 Haldane 模型 (R (2) = 1)。在分批操作和循环模式下,使用浓缩生物量研究了初始二乙酮浓度范围为 0.8-3.9 g/l 的二乙酮的生物降解。发现的生物降解速率遵循拟二级动力学,报告了得到的动力学参数。在所有测试的初始浓度下,去除率约为 100%,表明生物量浓度的增加可以在去除二乙酮方面取得更好的效果。这项研究表明,这些细菌非常有效地从水溶液中去除二乙酮。

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