Dieterlen-Lievre F, Jaffredo T, Bachnou N, al Moustafa A E, Saule S
Institut d'Embryologie Cellulaire et Moléculaire du CNRS, Nogent sur Marne, France.
Int J Dev Biol. 1990 Mar;34(1):61-8.
In order to detect signs of oncogene activity and elucidate their possible role in avian ontogeny we implemented two different strategies. One was to detect either the protein product or messenger RNA in situ at various stages of development. The other was to try and disturb development with retroviruses carrying one or several oncogenes in their activated forms. Time- and tissue-specific expression of c-myc was apparently not related to particular phases of cell evolution, such as population amplification. Rather the presence of c-myc immunoreactive product at particular stages appeared to depend on cell types. c-myb and c-ets messenger RNAs were found expressed preferentially in the blood system, respectively in hemopoietic and differentiating endothelial cells. The developing embryo heart was found to be uniquely sensitive to the effect of retroviruses provided that two conditions were respected. The first was the injection of the virus or construct prior to E3.5. The second was the presence of the v-myc gene, whether alone or associated with one or several other v-onc. In such cases a large proportion (70%) of chick and all quail embryos developed multiple heart rhabdomyosarcomas within 10 days. In chickens the association of a second v-onc or of two others induced the formation of secondary tumors, whose type was determined by the nature of the other oncogene(s).(ABSTRACT TRUNCATED AT 250 WORDS)
为了检测癌基因活性的迹象并阐明它们在禽类个体发育中的可能作用，我们实施了两种不同的策略。一种是在发育的各个阶段原位检测蛋白质产物或信使核糖核酸。另一种是尝试用携带一种或几种激活形式癌基因的逆转录病毒干扰发育。c-myc的时间和组织特异性表达显然与细胞进化的特定阶段无关，如群体扩增。相反，在特定阶段c-myc免疫反应性产物的存在似乎取决于细胞类型。发现c-myb和c-ets信使核糖核酸分别在造血系统和分化中的内皮细胞中优先表达。发现发育中的胚胎心脏对逆转录病毒的作用具有独特的敏感性，前提是满足两个条件。第一个条件是在胚胎发育3.5天之前注射病毒或构建体。第二个条件是存在v-myc基因，无论其单独存在还是与一个或几个其他v-癌基因相关联。在这种情况下，很大比例（70%）的鸡胚胎和所有鹌鹑胚胎在10天内会发生多发性心脏横纹肌肉瘤。在鸡中，第二个v-癌基因或另外两个v-癌基因的联合诱导了继发性肿瘤的形成，其类型由其他癌基因的性质决定。（摘要截短至250字）
