Krzyściak Pawel
Department of Mycology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, Poland.
Wiad Parazytol. 2011;57(2):107-10.
Candida albicans is a major human fungal pathogen especially as an etiologic agent of opportunistic oral and genital infections. Moreover, C. albicans can be involved in the deep infections and recent evidence suggests that the majority of diseases produced by this pathogen are associated with biofilm growth. The aims of this study were to evaluate biofilm production ability of C. albicans strains isolated from different sources, and to evaluate the effect of serum for enhancement the growth of biofilm. The strains used in this study were obtained from three sources; 12 from feces of patients with gastrointestinal disturbances, 13 from the oral cavity of patients with oral candidiasis, and 16 from the vagina of patients with Candida vulvovaginitis (CVV). Polystyrene 96-well plates were used to grow biofilms and crystal violet (CV) staining method was used to evaluate the growth. There were no differences in biofilm growth expressed as CV absorbance between C. albicans strains from different origins neither in Yeast Nitrogen Base broth (YNB) or in bovine serum (BS) (ANOVA, P = 0.1648, P = 0.5106, respectively). In the BS, the biofilm production was greater than in YNB medium for all samples (ANOVA, P = 0.0003).
白色念珠菌是一种主要的人类真菌病原体，尤其是作为机会性口腔和生殖器感染的病原体。此外，白色念珠菌可引发深部感染，最近的证据表明，该病原体引发的大多数疾病都与生物膜生长有关。本研究的目的是评估从不同来源分离的白色念珠菌菌株的生物膜形成能力，并评估血清对生物膜生长的促进作用。本研究中使用的菌株来自三个来源；12株来自胃肠道功能紊乱患者的粪便，13株来自口腔念珠菌病患者的口腔，16株来自念珠菌性外阴阴道炎（CVV）患者的阴道。使用聚苯乙烯96孔板培养生物膜，并采用结晶紫（CV）染色法评估生长情况。不同来源的白色念珠菌菌株在酵母氮源肉汤（YNB）或牛血清（BS）中，以CV吸光度表示的生物膜生长没有差异（方差分析，P分别为0.1648和0.5106）。在BS中，所有样品的生物膜形成均大于YNB培养基（方差分析，P = 0.0003）。
