Kirkpatrick W R, Lopez-Ribot J L, McAtee R K, Patterson T F
Departments of Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284, USA.
J Clin Microbiol. 2000 Feb;38(2):902-4. doi: 10.1128/JCM.38.2.902-904.2000.
Seven isolates each of Candida albicans and Candida dubliniensis were paired (11 pairs) and examined for competitive interaction. Equal numbers of CFU of each competitor were inoculated into Sabouraud dextrose broth and incubated at 37 degrees C with vigorous shaking under conditions favorable to either broth or biofilm growth. Surviving proportions of each competitor were calculated from the broth culture at 24 and 96 h and the biofilm culture at 96 h, with species differentiation done on CHROMagar Candida medium. C. albicans had a competitive advantage over C. dubliniensis in broth culture and under biofilm growing conditions; however, with the presence of a supporting structure for biofilm formation, C. dubliniensis was able to better withstand the competitive pressures from C. albicans.
将白色念珠菌和都柏林念珠菌各7个分离株进行配对（共11对），并检测其竞争相互作用。将等量的各竞争菌株的菌落形成单位接种到沙氏葡萄糖肉汤中，在有利于肉汤或生物膜生长的条件下，于37℃剧烈振荡培养。在24小时和96小时从肉汤培养物以及96小时从生物膜培养物中计算各竞争菌株的存活比例，并在CHROMagar念珠菌培养基上进行菌种鉴别。在肉汤培养和生物膜生长条件下，白色念珠菌比都柏林念珠菌具有竞争优势；然而，在存在生物膜形成的支持结构时，都柏林念珠菌能够更好地抵御来自白色念珠菌的竞争压力。
