两种不同受体的激活可从非洲爪蟾卵母细胞的不同储存部位动员钙。
Activation of two different receptors mobilizes calcium from distinct stores in Xenopus oocytes.
作者信息
Shapira H, Lupu-Meiri M, Gershengorn M C, Oron Y
机构信息
Division of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel.
出版信息
Biophys J. 1990 Jun;57(6):1281-5. doi: 10.1016/S0006-3495(90)82646-1.
Abstract
Acetylcholine (ACh) and thyrotropin-releasing hormone (TRH) utilize inositol 1,4,5-trisphosphate (IP3) as a second messenger and evoke independent depolarizing membrane electrical responses accompanied by characteristic 45Ca efflux profiles in Xenopus laevis oocytes injected with GH3 pituitary cell mRNA. To determine whether this could be accounted for by mobilization of calcium from functionally separate stores, we measured simultaneously 45Ca efflux and membrane electrical responses to ACh and TRH in single oocytes. We found that depletion of ACh-sensitive calcium store did not affect the membrane electrical response to TRH and the TRH-evoked 45Ca efflux. Our data suggest that ACh and TRH mobilize calcium from distinct cellular stores in the oocyte. This is the first demonstration in a single cell of strict subcellular compartmentalization of calcium stores coupled to two different populations of cell membrane receptors that utilize the same second messenger.
摘要
乙酰胆碱（ACh）和促甲状腺激素释放激素（TRH）利用肌醇1,4,5 - 三磷酸（IP3）作为第二信使，并在注射了GH3垂体细胞mRNA的非洲爪蟾卵母细胞中引发独立的去极化膜电反应，同时伴有特征性的45Ca流出曲线。为了确定这是否可归因于从功能上独立的储存库中动员钙，我们在单个卵母细胞中同时测量了45Ca流出以及对ACh和TRH的膜电反应。我们发现，ACh敏感钙储存库的耗尽并不影响对TRH的膜电反应以及TRH诱发的45Ca流出。我们的数据表明，ACh和TRH从卵母细胞中不同的细胞储存库中动员钙。这是在单个细胞中首次证明与利用相同第二信使的两种不同细胞膜受体群体相关的钙储存库的严格亚细胞区室化。
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Types of muscarinic response in Xenopus oocytes.非洲爪蟾卵母细胞中的毒蕈碱反应类型。
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Acetylcholine- and inositol 1,4,5-trisphosphate-induced calcium mobilization in Xenopus laevis oocytes.乙酰胆碱和肌醇1,4,5-三磷酸诱导非洲爪蟾卵母细胞中的钙动员。
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Mechanism of membrane electrical response to thyrotropin-releasing hormone in Xenopus oocytes injected with GH3 pituitary cell messenger ribonucleic acid.注射了GH3垂体细胞信使核糖核酸的非洲爪蟾卵母细胞中促甲状腺激素释放激素引起的膜电反应机制。
Mol Endocrinol. 1987 Dec;1(12):918-25. doi: 10.1210/mend-1-12-918.
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Differences in receptor-evoked membrane electrical responses in native and mRNA-injected Xenopus oocytes.天然和注射mRNA的非洲爪蟾卵母细胞中受体诱发的膜电反应差异。
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Coupling of inositol phospholipid hydrolysis to peptide hormone receptors expressed from adrenal and pituitary mRNA in Xenopus laevis oocytes.非洲爪蟾卵母细胞中肌醇磷脂水解与从肾上腺和垂体信使核糖核酸表达的肽激素受体的偶联。
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Decreased TRH receptor mRNA activity precedes homologous downregulation: assay in oocytes.促甲状腺激素释放激素（TRH）受体mRNA活性降低先于同源性下调：卵母细胞中的检测
Science. 1987 Dec 4;238(4832):1406-8. doi: 10.1126/science.2825350.
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Fluorescent probes of cell signaling.细胞信号传导的荧光探针。
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Receptor density determines secretory response patterns mediate by inositol lipid-derived second messengers. Comparison of thyrotropin-releasing hormone and carbamylcholine actions in thyroid-stimulating hormone-secreting mouse pituitary tumor cells.受体密度决定了由肌醇脂质衍生的第二信使介导的分泌反应模式。促甲状腺激素释放激素与氨甲酰胆碱在分泌促甲状腺激素的小鼠垂体瘤细胞中的作用比较。
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