来自GH4C1细胞的不同大小的信使核糖核酸在非洲爪蟾卵母细胞中诱导促甲状腺激素释放激素依赖的电反应。

Different-sized mRNAs from GH4C1 cells induce a TRH-dependent electrical response in Xenopus laevis oocytes.

作者信息

Wright M S, Karlsen H E, Paulssen E J, Meyerhof W, Høgset A, Gautvik K M

机构信息

Institute of Medical Biochemistry, University of Oslo, Norway.

出版信息

Acta Physiol Scand. 1990 Sep;140(1):129-34. doi: 10.1111/j.1748-1716.1990.tb08983.x.

DOI:10.1111/j.1748-1716.1990.tb08983.x

PMID:2177312

Abstract

Poly(A)+ RNA from the GH4C1 rat pituitary cell line elicited a thyrotropin releasing hormone response in Xenopus laevis oocytes which could be measured as a change in membrane current by the voltage-clamp method. Oocytes injected with Poly(A)+ RNA from GH12C1 cells which do not bind thyrotropin releasing hormone or with buffer solution alone did not show this response. Size fractionation of total poly(A)+ RNA by sucrose density-gradient centrifugation shows two response maximal representing various mRNA fractions larger than 18S. These results indicate the presence of thyrotropin releasing hormone receptor mRNA heterogeneity where the smallest mRNA is at least 2 kb.

摘要

来自GH4C1大鼠垂体细胞系的聚腺苷酸加尾（Poly(A)+）RNA在非洲爪蟾卵母细胞中引发了促甲状腺激素释放激素反应，该反应可通过电压钳法测量膜电流变化来检测。注射了来自不结合促甲状腺激素释放激素的GH12C1细胞的Poly(A)+ RNA或仅注射缓冲溶液的卵母细胞未显示出这种反应。通过蔗糖密度梯度离心对总聚腺苷酸加尾（Poly(A)+）RNA进行大小分级分离，结果显示有两个反应峰值，代表大于18S的各种mRNA组分。这些结果表明存在促甲状腺激素释放激素受体mRNA的异质性，其中最小的mRNA至少为2 kb。