State Key Laboratory of Reproductive Medicine, Institute of Toxicology, School of Public Health, Nanjing Medical University, China.
Clin Chim Acta. 2011 Sep 18;412(19-20):1778-82. doi: 10.1016/j.cca.2011.05.037. Epub 2011 Jun 13.
It is acknowledged that Cytochrome P450 1B1 (CYP1B1) plays a crucial role in metabolism and is involved in lots of diseases. We carried out this study to evaluate the association between CYP1B1 single nucleotide polymorphisms (SNPs) and male infertility in the Han-Chinese population with abnormal semen parameters.
We genotyped five CYP1B1 polymorphisms by using TaqMan allelic discrimination assay and Genome Lab SNP-stream. A total of 591 idiopathic infertile men and 419 fertile controls were comprised in the research. Semen quality analysis was performed using computer assisted sperm analysis. According to semen parameters, we divided cases into 3 subgroups in the stratified analysis.
In our study, we only found genetic variant rs1056836 is correlated with idiopathic male infertility (P=0.012). Additionally, in strategy analysis, rs1056836 may decrease the risk of abnormal sperm motility (OR=0.11, 95% CI=0.01, 0.86). While other four variants showed no significant association with male infertility.
Our results suggested that polymorphism of CYP1B1 modified the risk of male infertility, and men harboring this polymorphism had lower risk of abnormal sperm parameters. These findings should be validated by more epidemiological and functional studies.
细胞色素 P450 1B1（CYP1B1）在代谢中起着至关重要的作用，与许多疾病有关。我们进行这项研究是为了评估 CYP1B1 单核苷酸多态性（SNPs）与具有异常精液参数的汉族男性不育之间的关系。
我们使用 TaqMan 等位基因鉴别检测和 Genome Lab SNP-stream 对五个 CYP1B1 多态性进行了基因分型。共有 591 名特发性不育男性和 419 名生育能力正常的对照组参与了这项研究。使用计算机辅助精子分析进行精液质量分析。根据精液参数，我们在分层分析中对病例进行了 3 个亚组的划分。
在我们的研究中，我们只发现遗传变异 rs1056836 与特发性男性不育有关（P=0.012）。此外，在策略分析中，rs1056836 可能降低异常精子运动能力的风险（OR=0.11，95%CI=0.01，0.86）。而其他四个变体与男性不育没有显著关联。
我们的结果表明，CYP1B1 多态性改变了男性不育的风险，携带这种多态性的男性异常精子参数的风险较低。这些发现需要更多的流行病学和功能研究来验证。
