Endsley Michael P, Zhang Ming
Robert H. Lurie Comprehensive Cancer Center and Center for Genetic Medicine, Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, Feinberg School of Medicine, Chicago, Illinois, USA.
Methods Enzymol. 2011;499:149-65. doi: 10.1016/B978-0-12-386471-0.00008-0.
Clade B serpin family of proteins regulate a variety of cellular functions including cell adhesion and motility. One key member of the clade B serpin family is maspin (SERPINB5). Maspin is classified as a type II tumor suppressor that regulates cell adhesion and invasion. It is expressed in normal mammary epithelial cells but is reduced in benign breast tumors and absent in invasive breast carcinomas. Although maspin regulates cell apoptosis, cell adhesion, migration, and invasion in breast cancer cell culture systems, mouse models are necessary to verify this in vivo. In this chapter, we review the development of transgenic and syngeneic mouse models to study the role of maspin in mammary tumorigenesis and in normal mammary development.
B类丝氨酸蛋白酶抑制剂家族蛋白调节多种细胞功能，包括细胞黏附和运动。B类丝氨酸蛋白酶抑制剂家族的一个关键成员是乳腺丝抑蛋白（SERPINB5）。乳腺丝抑蛋白被归类为II型肿瘤抑制因子，可调节细胞黏附和侵袭。它在正常乳腺上皮细胞中表达，但在良性乳腺肿瘤中减少，在浸润性乳腺癌中缺失。尽管乳腺丝抑蛋白在乳腺癌细胞培养系统中调节细胞凋亡、细胞黏附、迁移和侵袭，但需要小鼠模型在体内进行验证。在本章中，我们综述了转基因和同基因小鼠模型的发展，以研究乳腺丝抑蛋白在乳腺肿瘤发生和正常乳腺发育中的作用。
