近红外反射光谱法预测了给饲亚麻籽的肉牛皮下脂肪中多不饱和脂肪酸和生物氢化产物的含量。

Near infrared reflectance spectroscopy predicts the content of polyunsaturated fatty acids and biohydrogenation products in the subcutaneous fat of beef cows fed flaxseed.

机构信息

Instituto de Ganadería de Montaña (Consejo Superior de Investigaciones Científicas, Universidad de León), Finca Marzanas, E-24346 Grulleros, León, Spain.

出版信息

Meat Sci. 2012 Jan;90(1):43-51. doi: 10.1016/j.meatsci.2011.05.025. Epub 2011 Jun 6.

DOI:10.1016/j.meatsci.2011.05.025

PMID:21683533

Abstract

This study examined the ability of near infrared reflectance spectroscopy (NIRS) to estimate the concentration of polyunsaturated fatty acids and their biohydrogenation products in the subcutaneous fat of beef cows fed flaxseed. Subcutaneous fat samples at the 12th rib of 62 cows were stored at -80°C, thawed, scanned over a NIR spectral range from 400 to 2498 nm at 31°C and 2°C, and subsequently analysed for fatty acid composition. Best NIRS calibrations were with samples at 31°C, showing high predictability for most of the n-3 (R(2): 0.81-0.86; RMSECV: 0.11-1.56 mg g(-1) fat) and linolenic acid biohydrogenation products such as conjugated linolenic acids, conjugated linoleic acids (CLA), non-CLA dienes and trans-monounsaturated fatty acids with R(2) (RMSECV, mgg(-1) fat) of 0.85-0.85 (0.16-0.37), 0.84-0.90 (0.21-2.58), 0.90 (5.49) and 0.84-0.90 (4.24-8.83), respectively. NIRS could discriminate 100% of subcutaneous fat samples from beef cows fed diets with and without flaxseed.

摘要

本研究旨在探讨近红外反射光谱（NIRS）在评估饲粮添加亚麻籽对奶牛皮下脂肪中多不饱和脂肪酸及其生物氢化产物浓度的能力。从 62 头奶牛的第 12 肋骨处采集皮下脂肪样本，在-80°C 下储存，于 31°C 和 2°C 下解冻，在 400-2498nm 的 NIR 光谱范围内扫描，并随后对脂肪酸组成进行分析。在 31°C 下对样本进行最佳 NIRS 校准，结果显示对大多数 n-3（R(2)：0.81-0.86；RMSECV：0.11-1.56mg g(-1) 脂肪）和亚麻酸生物氢化产物（如共轭亚油酸、共轭亚麻酸、非共轭亚油酸二烯和反式单不饱和脂肪酸）具有较高的预测能力，R(2)（RMSECV，mg g(-1) 脂肪）分别为 0.85-0.85（0.16-0.37）、0.84-0.90（0.21-2.58）、0.90（5.49）和 0.84-0.90（4.24-8.83）。NIRS 可以区分 100%饲粮中添加和未添加亚麻籽的奶牛的皮下脂肪样本。