Thayer School of Engineering at Dartmouth College, 8000 Cummings Hall, Hanover, NH 03755, USA.
Bioresour Technol. 2011 Sep;102(17):8040-5. doi: 10.1016/j.biortech.2011.05.021. Epub 2011 May 14.
In this study, efforts were taken to compare solubilization of Avicel and AFEX pretreated corn stover (AFEX CS) by SSF and Clostridium thermocellum fermentation, with an aim to gain insights into microbial conversion of pretreated cellulosic biomass. Solubilization rates for AFEX CS are comparable for the two systems while solubilization of Avicel is much faster by C. thermocellum. Initial catalyst loading impacts final cellulose conversion for SSF but not for C. thermocellum. Hydrolysis of the two substrates using cell-free C. thermocellum fermentation broth revealed much smaller difference in cellulose conversion than the difference observed for growing cultures. Tests on hemicellulose removal and particle size reduction for AFEX CS indicated that substrate accessibility is very important for enhanced solubilization by C. thermocellum.
在这项研究中，我们努力通过固态发酵和产热梭菌发酵来比较微晶纤维素和预处理玉米秸秆（AFEX CS）的增溶情况，旨在深入了解预处理纤维素生物质的微生物转化。对于这两个系统，AFEX CS 的增溶率是可比的，而产热梭菌对微晶纤维素的增溶速度要快得多。对于固态发酵，初始催化剂负载量会影响最终的纤维素转化率，但对于产热梭菌则不会。使用无细胞产热梭菌发酵液对这两种底物进行水解，发现纤维素转化率的差异比在生长培养物中观察到的差异要小得多。对 AFEX CS 的半纤维素去除和颗粒尺寸减小的测试表明，对于产热梭菌的增强增溶，底物的可及性非常重要。
