Arbor Fuel Inc., 11 Maple Vale Dr., Woodbridge, CT 06525, USA.
Bioresour Technol. 2011 Sep;102(17):8310-3. doi: 10.1016/j.biortech.2011.05.075. Epub 2011 May 30.
In this study an industrial Saccharomyces cerevisiae yeast strain capable of fermenting ethanol from pretreated lignocellulosic material was engineered. Genes encoding cellulases (endoglucanase, exoglucanase and β-glucosidase) were integrated into the chromosomal ribosomal DNA and delta regions of a derivative of the K1-V1116 wine yeast strain. The engineered cellulolytic yeast produces ethanol in one step through simultaneous saccharification and fermentation of pretreated biomass without the addition of exogenously produced enzymes. When ethanol fermentation was performed with 10% dry weight of pretreated corn stover, the recombinant strain fermented 63% of the cellulose in 96 h and the ethanol titer reached 2.6% v/v. These results demonstrate that cellulolytic S. cerevisiae strains can be used as a platform for developing an economical advanced biofuel process.
在这项研究中，对一株能够从预处理木质纤维素材料发酵生产乙醇的工业酿酒酵母菌株进行了工程改造。将纤维素酶（内切葡聚糖酶、外切葡聚糖酶和β-葡萄糖苷酶）的编码基因整合到 K1-V1116 葡萄酒酵母菌株的衍生菌株的染色体核糖体 DNA 和 δ 区。经过工程改造的纤维素分解酵母可以通过对预处理生物质的同步糖化和发酵，在不添加外源酶的情况下，一步法生产乙醇。当用 10%（干重）预处理玉米秸秆进行乙醇发酵时，重组菌株在 96 小时内发酵了 63%的纤维素，乙醇浓度达到 2.6%（v/v）。这些结果表明，纤维素分解酿酒酵母菌株可以用作开发经济高效的先进生物燃料工艺的平台。
