Doran-Peterson Joy, Jangid Amruta, Brandon Sarah K, DeCrescenzo-Henriksen Emily, Dien Bruce, Ingram Lonnie O
Microbiology Department, University of Georgia, Athens, GA, USA.
Methods Mol Biol. 2009;581:263-80. doi: 10.1007/978-1-60761-214-8_17.
Ethanol production by fermentation of lignocellulosic biomass-derived sugars involves a fairly ancient art and an ever-evolving science. Production of ethanol from lignocellulosic biomass is not avant-garde, and wood ethanol plants have been in existence since at least 1915. Most current ethanol production relies on starch- and sugar-based crops as the substrate; however, limitations of these materials and competing value for human and animal feeds is renewing interest in lignocellulose conversion. Herein, we describe methods for both simultaneous saccharification and fermentation (SSF) and a similar but separate process for partial saccharification and cofermentation (PSCF) of lignocellulosic biomass for ethanol production using yeasts or pentose-fermenting engineered bacteria. These methods are applicable for small-scale preliminary evaluations of ethanol production from a variety of biomass sources.
通过发酵木质纤维素生物质衍生的糖来生产乙醇涉及一门相当古老的技艺和一门不断发展的科学。从木质纤维素生物质生产乙醇并非前沿技术，木质乙醇工厂至少自1915年就已存在。当前大多数乙醇生产依赖淀粉和糖类作物作为底物；然而，这些原料的局限性以及在人类和动物饲料方面的竞争价值正重新激发人们对木质纤维素转化的兴趣。在此，我们描述了用于乙醇生产的同时糖化发酵（SSF）方法以及用于木质纤维素生物质部分糖化和共发酵（PSCF）的类似但独立的过程，该过程使用酵母或戊糖发酵工程菌。这些方法适用于对来自各种生物质来源的乙醇生产进行小规模初步评估。
