Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA.
Bioresour Technol. 2011 Sep;102(17):7850-9. doi: 10.1016/j.biortech.2011.05.043. Epub 2011 May 27.
Within the biorefinery paradigm, many non-monomeric sugar compounds have been shown to be inhibitory to enzymes and microbial organisms that are used for hydrolysis and fermentation. Here, two novel separation technologies, polyelectrolyte polymer adsorption and resin-wafer electrodeionization (RW-EDI), have been evaluated to detoxify a dilute acid pretreated biomass slurry. Results showed that detoxification of a dilute acid pretreated ponderosa pine slurry by sequential polyelectrolyte and RW-EDI treatments was very promising, with significant removal of acetic acid, 5-hydroxymethyl furfural, and furfural (up to 77%, 60%, and 74% removed, respectively) along with >97% removal of sulfuric acid. Removal of these compounds increased the cellulose conversion to 94% and elevated the hydrolysis rate to 0.69 g glucose/L/h. When using Saccharomyces cerevisiae D(5)A for fermentation of detoxified slurry, the process achieved 99% of the maximum theoretical ethanol yield and an ethanol production rate nearly five-times faster than untreated slurry.
在生物炼制范式中，已经证明许多非单体糖化合物对用于水解和发酵的酶和微生物生物体具有抑制作用。在这里，两种新型分离技术，聚电解质聚合物吸附和树脂晶圆电渗析（RW-EDI），已被评估用于解毒稀酸预处理生物质浆料。结果表明，通过顺序的聚电解质和 RW-EDI 处理，对稀酸预处理的黄松浆进行解毒非常有前景，可有效去除乙酸、5-羟甲基糠醛和糠醛（分别去除 77%、60%和 74%），同时硫酸去除率>97%。这些化合物的去除提高了纤维素转化率至 94%，并将水解速率提高至 0.69 g 葡萄糖/L/h。当使用酿酒酵母 D(5)A 发酵解毒后的浆料时，该工艺达到了最大理论乙醇产率的 99%，乙醇生产速率比未处理的浆料快近五倍。
