East China University of Science and Technology, Shanghai, China.
Appl Biochem Biotechnol. 2010 Jan;160(2):360-9. doi: 10.1007/s12010-008-8306-0. Epub 2008 Jul 15.
Steam-exploded corn stover biomass was used as the substrate for fed-batch separate enzymatic hydrolysis and fermentation (SHF) to investigate the solid concentration ranging from 10% to 30% (w/w) on the lignocellulose enzymatic hydrolysis and fermentation. The treatment of washing the steam-exploded material was also evaluated by experiments. The results showed that cellulose conversion changed little with increasing solid concentration, and fermentation by Saccharomyces cerevisiae revealed a nearly same ethanol yield with the water-washed steam-exploded corn stover. For the washed material at 30% substrate concentration, i.e., 30% water insoluble solids (WIS), enzymatic hydrolysis yielded 103.3 g/l glucose solution and a cellulose conversion of 72.5%, thus a high ethanol level up to 49.5 g/l. With the unwashed steam-exploded corn stover, though a cellulose conversion of 70.9% was obtained in hydrolysis at 30% solid concentration (27.9% WIS), its hydrolysate did not ferment at all, and the hydrolysate of 20% solid loading containing 3.3 g/l acetic acid and 145 mg/l furfural already exerted a strong inhibition on the fermentation and ethanol production.
蒸汽爆破玉米秸秆生物质被用作分批补料酶解和发酵（SHF）的底物，研究了从 10%到 30%（w/w）的固体浓度对木质纤维素酶解和发酵的影响。还通过实验评估了对蒸汽爆破物料进行洗涤的处理。结果表明，纤维素转化率随固体浓度的增加变化不大，酿酒酵母的发酵几乎与水洗蒸汽爆破玉米秸秆的乙醇产率相同。对于 30%底物浓度（即 30%水不溶性固体（WIS））的洗涤物料，酶解得到 103.3 g/l 的葡萄糖溶液和 72.5%的纤维素转化率，因此可达到高达 49.5 g/l 的高乙醇水平。对于未洗涤的蒸汽爆破玉米秸秆，尽管在 30%固体浓度（27.9%WIS）下的水解中获得了 70.9%的纤维素转化率，但它的水解液根本没有发酵，而含有 3.3 g/l 乙酸和 145 mg/l 糠醛的 20%固体负荷水解液已经对发酵和乙醇生产产生了强烈的抑制作用。
