Department of Molecular and Cellular Neurobiology, The Nencki Institute of Experimental Biology, Warsaw, Poland.
Exp Neurol. 2011 Sep;231(1):113-26. doi: 10.1016/j.expneurol.2011.05.022. Epub 2011 Jun 12.
Perineuronal nets (PNNs) are a condensed form of extracellular matrix that covers the surface of a subset of neurons. Their presence limits neuronal plasticity and may protect neurons against harmful agents. Here we analyzed the relationship between spatiotemporal changes in PNN expression and cell death markers after focal cortical photothrombotic stroke in rats. We registered a substantial decrease in PNN density using Wisteria floribunda agglutinin staining and CAT-315 and brevican immunoreactivity; the decrease occurred not only in the lesion core but also in the perilesional and remote cortex as well as in homotopic contralateral cortical regions. Fluoro Jade C and TUNEL staining in perilesional and remote areas, however, showed a low density of dying cells. Our results suggest that the PNN reduction was not a result of cellular death and could be considered an attempt to create conditions favorable for synaptic remodeling.
周围神经毡（PNNs）是一种浓缩的细胞外基质形式，覆盖在一部分神经元的表面。它们的存在限制了神经元的可塑性，并可能保护神经元免受有害物质的侵害。在这里，我们分析了大鼠局灶性皮质光血栓性中风后 PNN 表达和细胞死亡标志物的时空变化之间的关系。我们使用 Wisteria floribunda agglutinin 染色和 CAT-315 和 brevican 免疫反应，发现 PNN 密度有明显下降；这种下降不仅发生在病变核心区，也发生在病变周围和远隔皮层，以及同源对侧皮质区域。然而，在病变周围和远隔区域的氟罗丹明 C 和 TUNEL 染色显示，死亡细胞密度较低。我们的结果表明，PNN 的减少不是细胞死亡的结果，而可以被认为是一种创造有利于突触重塑的条件的尝试。
