The City College of New York, Department of Biomedical Engineering, New York, New York 10031, USA.
Nanomedicine. 2011 Dec;7(6):896-903. doi: 10.1016/j.nano.2011.05.002. Epub 2011 May 20.
Mechanistic study of biological processes via Quantum Dots (QDs) remain constrained by inefficient QD delivery methods and consequent altered cell function. Here the authors present a rapid method to label activated receptor populations in live cancer cells derived from medulloblastoma and glioma tumors. The authors used QDs to bind the extracellular domain of Epidermal Growth Factor Receptor (EGF-R) proteins and then induced receptor activation to facilitate specific detection of intracellular, activated EGF-R subpopulations. Such labeling enables rapid identification of biological markers characteristic of tumor type, grade and chemotherapy resistance.
In this paper, a rapid, quantum dot-based method is presented with the goal of labeling activated receptor populations in live cancer cells. More accurate characterization of medulloblastoma and glioma cancer cells using this biomarker detection technique may lead to a more specific targeted therapy.
通过量子点（QDs）对生物过程进行的机制研究仍然受到 QD 传递方法效率低下以及由此导致的细胞功能改变的限制。在这里，作者提出了一种快速标记来自成神经管细胞瘤和神经胶质瘤肿瘤的活癌细胞中激活受体群体的方法。作者使用 QD 结合表皮生长因子受体（EGF-R）蛋白的细胞外结构域，然后诱导受体激活，以促进细胞内、激活的 EGF-R 亚群的特异性检测。这种标记可以快速识别肿瘤类型、分级和化疗耐药性的生物标志物。
在本文中，提出了一种快速的基于量子点的方法，目的是标记活癌细胞中激活的受体群体。使用这种生物标志物检测技术更准确地表征成神经管细胞瘤和神经胶质瘤癌细胞，可能会导致更具针对性的靶向治疗。
