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Membrane association of the Tnp and Inh proteins of IS50R.

作者信息

DeLong A, Syvanen M

机构信息

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.

出版信息

J Bacteriol. 1990 Sep;172(9):5516-9. doi: 10.1128/jb.172.9.5516-5519.1990.

DOI:10.1128/jb.172.9.5516-5519.1990
PMID:2168388
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213226/
Abstract

Using a radioimmunoassay for the IS50R proteins Tnp and Inh, we found that both proteins were present primarily in the cytoplasm, but 3 to 11% of Tnp and 3 to 5% of Inh were found in association with the inner membrane. The fractions of total Tnp and Inh that became membrane bound were unaffected by the amount of Tnp and Inh synthesized in whole cells, provided that the ratio of total Tnp to total Inh was not changed. In addition, Inh was not found in the membrane fraction in Tnp- IS50R mutants, indicating that Tnp is required for Inh localization.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ab5/213226/796eb52fe472/jbacter00123-0788-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ab5/213226/2a8421b385f0/jbacter00123-0787-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ab5/213226/39882adcd310/jbacter00123-0787-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ab5/213226/796eb52fe472/jbacter00123-0788-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ab5/213226/2a8421b385f0/jbacter00123-0787-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ab5/213226/39882adcd310/jbacter00123-0787-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ab5/213226/796eb52fe472/jbacter00123-0788-a.jpg

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引用本文的文献

1
Examination of the Tn5 transposase overproduction phenotype in Escherichia coli and localization of a suppressor of transposase overproduction killing that is an allele of rpoH.大肠杆菌中Tn5转座酶过量表达表型的检测以及作为rpoH等位基因的转座酶过量表达致死抑制子的定位。
J Bacteriol. 1997 Mar;179(5):1704-13. doi: 10.1128/jb.179.5.1704-1713.1997.
2
Overexpression of the Tn5 transposase in Escherichia coli results in filamentation, aberrant nucleoid segregation, and cell death: analysis of E. coli and transposase suppressor mutations.Tn5转座酶在大肠杆菌中的过表达导致丝状化、异常的类核分离和细胞死亡：大肠杆菌及转座酶抑制突变分析
J Bacteriol. 1994 Sep;176(17):5494-504. doi: 10.1128/jb.176.17.5494-5504.1994.
3

本文引用的文献

1
The tac promoter: a functional hybrid derived from the trp and lac promoters.Tac启动子：一种源自色氨酸启动子和乳糖启动子的功能性杂种启动子。
Proc Natl Acad Sci U S A. 1983 Jan;80(1):21-5. doi: 10.1073/pnas.80.1.21.
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Control of Tn5 transposition in Escherichia coli is mediated by protein from the right repeat.大肠杆菌中Tn5转座的控制由来自右侧重复序列的蛋白质介导。
Trans-acting transposase mutant from Tn5.
来自Tn5的反式作用转座酶突变体
Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6072-6. doi: 10.1073/pnas.88.14.6072.
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Fis plays a role in Tn5 and IS50 transposition.Fis在Tn5和IS50转座过程中发挥作用。
J Bacteriol. 1992 Jul;174(14):4530-7. doi: 10.1128/jb.174.14.4530-4537.1992.
6
Characterization of two hypertransposing Tn5 mutants.两种高转座Tn5突变体的表征
J Bacteriol. 1992 Feb;174(4):1229-39. doi: 10.1128/jb.174.4.1229-1239.1992.
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Structural analysis of Tn5.Tn5的结构分析
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5
The functional differences in the inverted repeats of Tn5 are caused by a single base pair nonhomology.Tn5反向重复序列中的功能差异是由单个碱基对的非同源性引起的。
Cell. 1981 Jan;23(1):191-9. doi: 10.1016/0092-8674(81)90284-1.
6
Role of the IS50 R proteins in the promotion and control of Tn5 transposition.IS50 R蛋白在Tn5转座促进与调控中的作用。
J Mol Biol. 1984 Aug 25;177(4):645-61. doi: 10.1016/0022-2836(84)90042-1.
7
DNA methylation in Escherichia coli.大肠杆菌中的DNA甲基化
Annu Rev Genet. 1987;21:113-31. doi: 10.1146/annurev.ge.21.120187.000553.
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Compartmentalization of the proteins encoded by IS50R.
J Biol Chem. 1985 Mar 25;260(6):3645-51.
9
p2 and inhibition of Tn5 transposition.p2与Tn5转座的抑制作用。
J Bacteriol. 1988 Jul;170(7):3008-15. doi: 10.1128/jb.170.7.3008-3015.1988.
10
Effect of dam methylation on Tn5 transposition.DNA甲基化对Tn5转座的影响。
J Mol Biol. 1988 Jan 5;199(1):35-45. doi: 10.1016/0022-2836(88)90377-4.