Am J Bot. 1998 Mar;85(3):352.
In order to test the "anti-interference" hypothesis for adaptive significance of reciprocal herkogamy, patterns of illegitimate pollination and effects of self-pollen on the legitimate pollination and fertility were investigated in a naturally pollinated experimental population of distylous Persicaria japonica. Pollen deposition was compared among the emasculation treatments, i.e., emasculation of a single flower from individual inflorescences, emasculation of all the flowers of individual inflorescences, and no emasculation control. In both morphs, considerable illegitimate pollination was found to occur in all the treatments, and there was no significant difference in incompatible pollen load among the treatments. Therefore, it is suggested that herkogamy of P. japonica can sufficiently reduce both intraflower and intra-inflorescence self-pollinations, but not interinflorescence geitonogamous and-or interclonal illegitimate pollination. Measurements of pollen load after the repetitive pollen addition showed that space on the stigma surface may not limit the legitimate pollination under natural pollination conditions. Seed sets after legitimate pollination following prior self-pollination did not differ from the controls without self-pollination. Therefore, there is little possibility that incompatible pollen load interferes with either pollination or fertilization by compatible pollen, suggesting that "anti-interference" is unimportant for adaptive significance of reciprocal herkogamy at least in P. japonica.
为了检验雌雄异位的“抗干扰”假说对于适应意义，我们在自然授粉的二型日本蓼实验种群中研究了非法授粉的模式及其自花粉对合法授粉和结实的影响。我们比较了三种去雄处理的花粉沉积情况，即从个体花序上的一朵花去雄、从个体花序上的所有花去雄和不进行去雄对照。在两种花型中，所有处理都发现了相当多的非法授粉，而且处理之间的不亲和花粉负荷没有显著差异。因此，日本蓼的雌雄异位可以充分减少同花内和花序内的自授粉，但不能减少花序间的异交和/或克隆间的非法授粉。重复添加花粉后的花粉负荷测量表明，在自然授粉条件下，柱头表面的空间可能不会限制合法授粉。自授粉后进行合法授粉的结实率与未进行自授粉的对照没有差异。因此，不亲和花粉负荷不太可能干扰亲和花粉的授粉或受精，这表明在至少在日本蓼中，“抗干扰”对于雌雄异位的适应意义不重要。
