Anderson R J, Cambria R, Kerr J, Hobson R W
University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103-2757.
J Surg Res. 1990 Sep;49(3):271-5. doi: 10.1016/0022-4804(90)90132-l.
Limiting the rate of reperfusion blood flow following prolonged ischemia in skeletal muscle has been shown beneficial. However, the persistence of this benefit with reinstitution of normal blood flow remains undefined. We investigated the role of temporary limited reperfusion on ischemia-reperfusion injury in an isolated gracilis muscle model in six anesthetized dogs. Both gracilis muscles were subjected to 6 hr of ischemia followed by 2 hr of reperfusion. Reperfusion blood flow was limited for the first hour in one gracilis muscle to its preischemic rate followed by a second hour of normal reperfusion (LR/NR). The contralateral muscle underwent 2 hr of normal reperfusion (NR/NR). Muscle injury was quantified by technetium-99m pyrophosphate (TcPyp) uptake and by histochemical staining using triphenyltetrazolium chloride (TTC) with planimetry of the infarct size. Capillary permeability was evaluated by muscle weight gain. Results are reported as the mean +/- SEM: [table: see text] These data demonstrate a sustained benefit from temporary limited reperfusion. This methodology should be considered in the surgical management of the acutely ischemic limb.
在骨骼肌长时间缺血后限制再灌注血流速率已被证明是有益的。然而，恢复正常血流后这种益处的持续性仍不明确。我们在六只麻醉犬的离体股薄肌模型中研究了临时有限再灌注对缺血再灌注损伤的作用。双侧股薄肌均经历6小时缺血，随后进行2小时再灌注。一侧股薄肌在再灌注的第一小时将血流限制在缺血前速率，随后第二小时进行正常再灌注（LR/NR）。对侧肌肉进行2小时正常再灌注（NR/NR）。通过99m锝焦磷酸盐（TcPyp）摄取以及使用氯化三苯基四氮唑（TTC）进行组织化学染色并通过梗死面积的平面测量法对肌肉损伤进行定量。通过肌肉重量增加评估毛细血管通透性。结果报告为平均值±标准误：[表格：见原文] 这些数据表明临时有限再灌注具有持续的益处。在急性缺血肢体的外科治疗中应考虑这种方法。
