Bakke H K, Bogsnes A, Murison R
Institute of Physiological Psychology, University of Bergen, Norway.
Physiol Behav. 1990 Jun;47(6):1253-60. doi: 10.1016/0031-9384(90)90379-i.
The role of an intact locus coeruleus (LC) noradrenergic system for the central effects of corticotropin-releasing factor (CRF) was studied. Rats were treated with N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP-4), a highly selective noradrenergic neurotoxin permanently affecting mainly the LC system. This procedure did not affect the activation of the sympathetic nervous system, as measured by plasma catecholamine levels, after either intracerebroventricular (ICV) or intraperitoneal (IP) administration of 8-micrograms CRF. Neither was the increased emotionality seen in an open field test after ICV injection of CRF altered. However, the ulceroprotective effect of 8-micrograms CRF ICV during a 2-hr water restraint stress was significantly antagonized by pretreatment with DSP-4, while CRF did exhibit an ulceroprotective effect after IP administration to DSP-4-treated animals. Our data indicate that the ulceroprotective effect of CRF under restraint stress is, at least partly, dependent on an intact locus coeruleus noradrenergic system.
研究了完整的蓝斑（LC）去甲肾上腺素能系统对促肾上腺皮质激素释放因子（CRF）中枢效应的作用。用盐酸N-（2-氯乙基）-N-乙基-2-溴苄胺（DSP-4）处理大鼠，DSP-4是一种高度选择性的去甲肾上腺素能神经毒素，主要永久性地影响LC系统。该操作不影响通过血浆儿茶酚胺水平测量的交感神经系统的激活，无论是在脑室内（ICV）还是腹腔内（IP）注射8微克CRF后。ICV注射CRF后在旷场试验中观察到的情绪增加也没有改变。然而，在2小时禁水应激期间，8微克CRF ICV的抗溃疡作用被DSP-4预处理显著拮抗，而CRF在给DSP-4处理的动物进行IP给药后确实表现出抗溃疡作用。我们的数据表明，CRF在束缚应激下的抗溃疡作用至少部分依赖于完整的蓝斑去甲肾上腺素能系统。
