Pan Pinhua, Huang Siyun, Hu Chengping
Department of Respiratory Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 May;36(5):386-91. doi: 10.3969/j.issn.1672-7347.2011.05.003.
To investigate the mechanism of airway neurogenic inflammation by studying the expression of inducible nitric oxide synthase (iNOS) and substance P in C(7)-T(5) dorsal root ganglion sensory neuron cells induced by nerve growth factor (NGF) and the role of interferon regulatory factor-1 (IRF-1).
The dorsal root ganglia neuron (DRGn) cells were primary cultured, and then stimulated with or without NGF or NGF+interferon (IFN)-γ. Subsequently the DRGn cells were transinfected with or without green fluorescent protein (GFP)-IRF-1-vshRNA, and then stimulated with or without NGF. The expressions of iNOS and substance P were detected by real-time PCR.
NGF induced the mRNA expression of iNOS and substance P in dorsal root ganglion sensory neuron cells, and IFN-γ increased NGF-induced iNOS mRNA expression. The expressions of iNOS and substance P in sensory neuron cells were decreased significantly at the mRNA level after IRF-1 was blocked down by IRF-1-vshRNA transinfection.
NGF is involved in the airway neurogenic inflammation by prompting the expression of iNOS and substance P through transcription factor IRF-1 in airway sensory neuron cells. IRF-1 may be a therapeutic target for airway neurogenic inflammation.
通过研究神经生长因子（NGF）诱导的C（7）-T（5）背根神经节感觉神经元细胞中诱导型一氧化氮合酶（iNOS）和P物质的表达以及干扰素调节因子-1（IRF-1）的作用，探讨气道神经源性炎症的机制。
原代培养背根神经节神经元（DRGn）细胞，然后用或不用NGF或NGF+干扰素（IFN）-γ刺激。随后，用或不用绿色荧光蛋白（GFP）-IRF-1-vshRNA转染DRGn细胞，然后用或不用NGF刺激。通过实时PCR检测iNOS和P物质的表达。
NGF诱导背根神经节感觉神经元细胞中iNOS和P物质的mRNA表达，IFN-γ增加NGF诱导的iNOS mRNA表达。通过IRF-1-vshRNA转染阻断IRF-1后，感觉神经元细胞中iNOS和P物质的mRNA水平显著降低。
NGF通过转录因子IRF-1促进气道感觉神经元细胞中iNOS和P物质的表达，参与气道神经源性炎症。IRF-1可能是气道神经源性炎症的治疗靶点。
