Enrich C, Evans W H
Departamento de Biología Celular, Facultad de Medicina, Universidad de Barcelona, España.
Rev Esp Fisiol. 1990 Mar;46(1):95-101.
When many ligands, polypeptidic hormones, growth factors, metabolic carriers, plasma glycoproteins, etc., bind to cell-surface receptors, ligand-receptor complexes are internalized by a process called receptor-mediated endocytosis towards the endocytic compartment. The endocytic compartment is an extensive network of anastomosing vesiculo-tubular membranes that differs biochemical and functionally from other intracellular organelles. Endosome fractions were prepared and antibodies raised against endosome membrane proteins. In addition to a detailed biochemical study of proteins and glycoproteins the antibodies were used to immunolocalize the endocytic structures in the hepatic cell. These studies aided to demonstrate the involvement of endocytic compartment not only in the sorting of proteins to specific domains of the plasma membrane but in the identification of "resident" endosome components.
当许多配体，如多肽激素、生长因子、代谢载体、血浆糖蛋白等与细胞表面受体结合时，配体 - 受体复合物通过一种称为受体介导的内吞作用的过程被内化，进入内吞区室。内吞区室是一个由相互吻合的囊泡 - 管状膜组成的广泛网络，在生化和功能上与其他细胞内细胞器不同。制备了内体组分，并制备了针对内体膜蛋白的抗体。除了对蛋白质和糖蛋白进行详细的生化研究外，这些抗体还用于免疫定位肝细胞中的内吞结构。这些研究有助于证明内吞区室不仅参与蛋白质向质膜特定结构域的分选，还参与“驻留”内体组分的鉴定。
