Department of Stomatology, Nanfang Hospital, College of Stomatology, Southern Medical University, Guangzhou, PR China.
Int J Mol Med. 2011 Oct;28(4):659-67. doi: 10.3892/ijmm.2011.721. Epub 2011 Jun 15.
Dentin sialophosphoprotein (DSPP), an important marker of odontoblast differentiation, is a prerequisite for tooth development and mineralization; however, the molecular mechanisms of both temporal and spatial regulation remain unknown. MicroRNAs (miRNAs) provide an additional level of control beyond that of transcription factors, which regulate post-transcriptional control of gene expression. The present study was designed to provide a first attempt at an in-depth analysis of dental pulp cells at various odontoblastic differentiation stages to obtain miRNA differential expression patterns, and to determine the contribution of miRNAs in the expression of DSPP during odontoblast differentiation. Dual luciferase reporter assays and qRT-PCR were used to validate miRNAs identified from bioinformatic analyses to determine whether they were able to regulate the DSPP gene in dental pulp cells cultured in a mineralizing medium. The results presented here suggest that DSPP is regulated post-transcriptionally by mir32, mir885-5p and mir586 during odontoblast differentiation.
牙本质涎磷蛋白（DSPP）是牙本质细胞分化的重要标志物，是牙齿发育和矿化的前提条件；然而，其时空调控的分子机制尚不清楚。微小 RNA（miRNA）在转录因子调控基因表达的转录后水平上提供了额外的调控水平。本研究旨在首次对不同牙本质分化阶段的牙髓细胞进行深入分析，以获得 miRNA 差异表达谱，并确定 miRNA 在牙本质细胞分化过程中对 DSPP 表达的贡献。双荧光素酶报告基因检测和 qRT-PCR 用于验证从生物信息学分析中鉴定出的 miRNA，以确定它们是否能够调节矿化培养基中培养的牙髓细胞中的 DSPP 基因。这里提出的结果表明，miR32、miR885-5p 和 miR586 在牙本质细胞分化过程中通过转录后调控 DSPP 的表达。
