School of Life Sciences, Gwangju Institute of Science Technology, Gwangju, Republic of Korea.
FEBS Lett. 2011 Jul 21;585(14):2323-30. doi: 10.1016/j.febslet.2011.05.069. Epub 2011 Jun 16.
The lateral diffusion of BK(Ca) channels was previously shown to be highly 'confined' in the COS-7 cell membrane. Here we report that the diffusion coefficient and the confinement area of BK(Ca) channel were significantly increased by the treatment of latrunculin A, an actin-depolymerizing agent, but not by microtubule disruption. Site-directed mutational analyses further demonstrated that a single leucine residue in the C-terminal actin-binding motif was critical for the aforementioned effects of latrunculin A. We conclude that some BK(Ca) channels are directly associated with actin filaments and their lateral mobility can be restricted by the cytoskeletal components.
BK(Ca) 通道的侧向扩散先前被证明在 COS-7 细胞膜中高度“受限”。在这里，我们报告说，有丝分裂蛋白 A（一种肌动蛋白解聚剂）处理可显著增加 BK(Ca) 通道的扩散系数和限制面积，但微管破坏则没有。定点突变分析进一步表明，在 C 末端肌动蛋白结合基序中的单个亮氨酸残基对于 latrunculin A 的上述作用至关重要。我们得出结论，一些 BK(Ca) 通道直接与肌动蛋白丝相关联，其侧向流动性可受到细胞骨架成分的限制。
