胡宁病毒田间分离株的病毒多样性。

Viral diversity of Junín virus field strains.

机构信息

Laboratorio de Ingeniería Genética y Biología Celular y Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD, Bernal, Buenos Aires, Argentina.

出版信息

Virus Res. 2011 Sep;160(1-2):150-8. doi: 10.1016/j.virusres.2011.06.004. Epub 2011 Jun 13.

DOI:10.1016/j.virusres.2011.06.004

PMID:21689697

Abstract

The Argentine Hemorrhagic Fever, an endemic disease present in a much of Argentina, is caused by the Junín virus (JUNV). Currently, there are sequences available from several strains of this virus, like those belonging to the vaccine lineage (XJ13, XJ#44 and Candid#1), as well as MC2 (rodent isolate) and IV4454 (human isolate). In this article, we report sequence information on two fragments of genomic segment S of viral isolates from the endemic area. A Nested-RT-PCR was used to amplify discrete genomic regions of 13 isolates of rodent and human origin. The bioinformatics studies revealed a great homogeneity of sequences among the JUNV isolates. The phylogenetic classification showed greater evolutionary distance between the old world arenaviruses (Lassa and LCM virus) than between the new world arenaviruses (JUNV and Machupo virus).

摘要

阿根廷出血热是一种在阿根廷大部分地区流行的地方性疾病，由胡宁病毒（JUNV）引起。目前，已经有来自该病毒的多个毒株的序列，如属于疫苗谱系的毒株（XJ13、XJ#44 和 Candid#1），以及 MC2（啮齿动物分离株）和 IV4454（人类分离株）。在本文中，我们报告了来自流行地区的病毒分离株基因组片段 S 的两个片段的序列信息。使用巢式 RT-PCR 扩增了 13 株来自啮齿动物和人类的分离株的离散基因组区域。生物信息学研究表明，JUNV 分离株之间的序列高度同源。系统发育分类显示，旧世界沙粒病毒（拉沙病毒和 LCM 病毒）之间的进化距离大于新世界沙粒病毒（JUNV 和马丘波病毒）之间的进化距离。