使用单体亲和素可重复富集血清细胞外热休克蛋白。
Reproducible enrichment of extracellular heat shock proteins from blood serum using monomeric avidin.
机构信息
Department of Chemistry, Hunter College of CUNY, 695 Park Avenue, New York, NY 10065, USA.
出版信息
Bioorg Med Chem Lett. 2011 Jul 15;21(14):4134-7. doi: 10.1016/j.bmcl.2011.05.111. Epub 2011 Jun 6.
Abstract
Extracellular heat shock proteins (eHsps) in blood circulation have been associated with various diseases, including cancer. However, the lack of methods to enrich eHsps from serum samples has hampered the characterization of eHsps. This Letter presents our serendipitous finding that the monomeric avidin resin can serve as an affinity resin to enrich eHsps from blood serum. Biochemical mechanism of this eHsp enrichment as well as implications in biomarker discovery is discussed.
摘要
细胞外热休克蛋白（eHsps）在血液循环中与各种疾病有关，包括癌症。然而，缺乏从血清样本中富集 eHsps 的方法，这阻碍了对 eHsps 的特征描述。本研究报告了一个偶然的发现，即单体亲和素树脂可用作亲和树脂，从血清中富集 eHsps。讨论了这种 eHsp 富集的生化机制及其在生物标志物发现中的意义。
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