Università degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Via Celoria 10, 20133 Milano, Italy.
Vet J. 2012 Apr;192(1):126-8. doi: 10.1016/j.tvjl.2011.05.001.
The in vitro permissivity to infection with homologous and heterologous bovine viral diarrhoea virus (BVDV) strains of bovine peripheral blood mononuclear cells (PBMCs) from eight naïve and eight BVDV-1b immune animals was studied. Four reference strains (BVDV-1a NADL, BVDV-1b NY-1, BVDV-2 125 and BVDV-2 890) were selected, based on genotype, prevalence and biotype. Virus neutralizing antibody titres were determined at bleeding and the viral loads were measured in PBMCs by end point titration in cell culture and by real-time PCR. PBMCs from both naïve and immune animals became infected by all BVDV strains tested, although virus titres were lower for immune heifers than naïve ones; the differences were significant for NADL (P<0.05) and 890 (P<0.001) strains. The in vitro model used in this study showed that PBMCs from immune animals are susceptible to re-infection with both homologous and heterologous BVDV strains, albeit at a lower extent than naïve cattle.
本研究旨在评估 8 头未经感染的牛和 8 头牛病毒性腹泻病毒（BVDV）1b 型免疫牛的外周血单个核细胞（PBMCs）对同源和异源 BVDV 毒株感染的体外易感性。基于基因型、流行率和生物型，选择了 4 株参考株（BVDV-1a NADL、BVDV-1b NY-1、BVDV-2 125 和 BVDV-2 890）。在采血时测定病毒中和抗体滴度，并通过细胞培养终点滴定和实时 PCR 测量 PBMCs 中的病毒载量。尽管免疫小母牛的病毒滴度低于未经感染的小母牛，但所有测试的 BVDV 株都能感染来自未经感染和免疫动物的 PBMCs；对于 NADL（P<0.05）和 890（P<0.001）株，差异具有统计学意义。本研究中使用的体外模型表明，免疫动物的 PBMCs易再次感染同源和异源 BVDV 株，但程度低于未经感染的牛。
