Lin Tian, Yu Jiang, Wei Zhi-gang, Hu Yan-feng, Wang Ya-nan, Li Guo-xin
Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jun;31(6):1066-8.
To evaluate the effect of simulated intraperitoneal 5-fluorouracil (5-Fu) aerosol chemotherapy (AIPC) on the proliferation, apoptosis, and cell cycle of human gastric cancer cell line MKN-45 in vitro.
The gastric cancer cells MKN-45 were treated with 5-Fu aerosol for 30 min under the pressure of 8 mmHg, and those treated with normal saline (NS) aerosol served as the control. The cell proliferation after the treatment was detected by MTT assay, and flow cytometry and FITC Annexin V/PI kit were used to detect the cell apoptosis and changes in the cell cycle.
MTT assay showed a significantly greater inhibition rate of the cell proliferation in 5-Fu aerosol group than in NS group [(31.13∓3.51)% vs (4.65∓1.99)%, P<0.001]. FCM analysis also showed a significantly higher cell apoptotic rate in 5-Fu aerosol group than in NS group [(12.00∓0.92)% vs (2.65∓0.52)%, P<0.001]. Compared with saline treatment, treatment with 5-Fu aerosol resulted in a greater proportion of G1 phase cells [(51.83∓1.95)% vs (36.41∓2.33)%, P<0.001] with a lowered proportion of S phase cells [(16.72∓2.36)% vs (45.20∓3.27)%, P<0.001].
Simulated 5-Fu AIPC can inhibit the proliferation, induce cell apoptosis and cause cell cycle arrest at G1 phase in gastric cancer cells.
评估模拟腹腔内5-氟尿嘧啶（5-Fu）雾化化疗（AIPC）对人胃癌细胞系MKN-45体外增殖、凋亡及细胞周期的影响。
将胃癌细胞MKN-45在8 mmHg压力下用5-Fu气雾剂处理30分钟，以用生理盐水（NS）气雾剂处理的细胞作为对照。处理后通过MTT法检测细胞增殖情况，采用流式细胞术和FITC Annexin V/PI试剂盒检测细胞凋亡及细胞周期变化。
MTT法显示，5-Fu气雾剂组细胞增殖抑制率显著高于NS组[（31.13±3.51）%对（4.65±1.99）%，P<0.001]。流式细胞术分析还显示，5-Fu气雾剂组细胞凋亡率显著高于NS组[（12.00±0.92）%对（2.65±0.52）%，P<0.001]。与盐水处理相比，5-Fu气雾剂处理导致G1期细胞比例更高[（51.83±1.95）%对（36.41±2.33）%，P<0.001]，S期细胞比例降低[（16.72±2.36）%对（45.20±3.27）%，P<0.001]。
模拟5-Fu AIPC可抑制胃癌细胞增殖，诱导细胞凋亡并使细胞周期停滞于G1期。
