Tang Cai, Zelenak Christine, Völkl Jakob, Eichenmüller Melanie, Regel Ivonne, Fröhlich Henning, Kempe Daniela, Jimenez Liliana, Le Bellego Laurent, Vergne Sebastien, Lang Florian
Department of Physiology, University of Tübingen, Gmelinstr. 5, D-72076 Tübingen.
Cell Physiol Biochem. 2011;27(6):757-68. doi: 10.1159/000330084. Epub 2011 Jun 17.
Dehydration has a profound influence on neuroexcitability. The mechanisms remained, however, incompletely understood. The present study addressed the effect of water deprivation on gene expression in the brain. To this end, animals were exposed to a 24 hours deprivation of drinking water and neuronal gene expression was determined by microarray technology with subsequent confirmation by RT-PCR. As a result, water deprivation was followed by significant upregulation of clathrin (light polypeptide Lcb), serum/glucocorticoid-regulated kinase (SGK) 1, and protein kinase A (PRKA) anchor protein 8-like. Water deprivation led to downregulation of janus kinase and microtubule interacting protein 1, neuronal PAS domain protein 4, thrombomodulin, purinergic receptor P2Y - G-protein coupled 13 gene, gap junction protein beta 1, neurotrophin 3, hyaluronan and proteoglycan link protein 1, G protein-coupled receptor 19, CD93 antigen, forkhead box P1, suppressor of cytokine signaling 3, apelin, immunity-related GTPase family M, serine (or cysteine) peptidase inhibitor clade B member 1a, serine (or cysteine) peptidase inhibitor clade H member 1, glutathion peroxidase 8 (putative), discs large (Drosophila) homolog-associated protein 1, zinc finger and BTB domain containing 3, and H2A histone family member V. Western blotting revealed the downregulation of forkhead box P1, serine (or cysteine) peptidase inhibitor clade H member 1, and gap junction protein beta 1 protein abundance paralleling the respective alterations of transcript levels. In conclusion, water deprivation influences the transcription of a wide variety of genes in the brain, which may participate in the orchestration of brain responses to water deprivation.
脱水对神经兴奋性有深远影响。然而，其机制仍未完全明确。本研究探讨了禁水对大脑基因表达的影响。为此，将动物暴露于24小时禁水状态，通过微阵列技术测定神经元基因表达，随后用逆转录聚合酶链反应进行确认。结果显示，禁水后网格蛋白（轻链多肽Lcb）、血清/糖皮质激素调节激酶（SGK）1和蛋白激酶A（PRKA）锚定蛋白8样蛋白显著上调。禁水导致janus激酶、微管相互作用蛋白1、神经元PAS结构域蛋白4、血栓调节蛋白、嘌呤能受体P2Y - G蛋白偶联13基因、缝隙连接蛋白β1、神经营养因子3、透明质酸和蛋白聚糖连接蛋白1、G蛋白偶联受体19、CD93抗原、叉头框P1、细胞因子信号转导抑制因子3、apelin、免疫相关GTP酶家族M、丝氨酸（或半胱氨酸）肽酶抑制剂B家族成员1a、丝氨酸（或半胱氨酸）肽酶抑制剂H家族成员1、谷胱甘肽过氧化物酶8（推测）、盘状大蛋白（果蝇）同源物相关蛋白1、锌指和BTB结构域包含蛋白3以及H2A组蛋白家族成员V下调。蛋白质印迹法显示叉头框P1、丝氨酸（或半胱氨酸）肽酶抑制剂H家族成员1和缝隙连接蛋白β1蛋白丰度下调，与转录水平的相应变化一致。总之，禁水影响大脑中多种基因的转录，这些基因可能参与大脑对禁水反应的调控。
