IntegenX, 5720 Stoneridge Drive, Pleasanton, CA 94588, USA.
Lab Chip. 2011 Aug 7;11(15):2541-50. doi: 10.1039/c1lc20244h. Epub 2011 Jun 21.
We describe a programmable microfluidic system with onboard pumps and valves that has the ability to process reaction volumes in the sub-microlitre to hundred microlitre range. The flexibility of the architecture is demonstrated with a commercial molecular biology protocol for mRNA amplification, implemented without significant modification. The performance of the microchip system is compared to conventional bench processing at each stage of the multistep protocol, and DNA microarrays are used to assess the quality and performance of bench- and microchip-amplified RNA. The results show that the microchip system reactions are similar to bench control reactions at each step, and that the microchip- and bench-derived amplified RNAs are virtually indistinguishable in differential microarray analyses.
我们描述了一种可编程微流控系统，该系统具有内置的泵和阀，能够处理亚微升至数百微升范围内的反应体积。通过商业分子生物学 mRNA 扩增协议的实施，证明了该架构的灵活性，而无需进行重大修改。该微芯片系统的性能与多步协议的每个阶段的传统台式处理进行了比较，并且 DNA 微阵列用于评估台式和微芯片扩增 RNA 的质量和性能。结果表明，微芯片系统反应在每个步骤都类似于台式对照反应，并且微芯片和台式衍生的扩增 RNA 在差异微阵列分析中几乎无法区分。
