Department of Chemistry, The University of Burdwan, Burdwan, 713104, West Bengal, India.
Org Biomol Chem. 2011 Aug 7;9(15):5523-9. doi: 10.1039/c1ob05479a. Epub 2011 Jun 21.
An efficient fluorescent Al(3+) receptor, N-(2-hydroxy-1-naphthalene)-N'-(2-(2-hydroxy-1-naphthalene)amino-ethyl)-ethane-1,2-diamine (L) has been synthesized by the condensation reaction between 2-hydroxy naphthaldehyde and diethylenetriamine. High selectivity and affinity of L towards Al(3+) in ethanol (EtOH) as well as in HEPES buffer at pH 7.4, makes it suitable to detect intracellular Al(3+) with fluorescence microscopy. Metal ions, viz. Li(+), Na(+), K(+), Mg(2+), Ca(2+), Mn(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Ag(+), Cd(2+), Hg(2+) and Pb(2+) do not interfere. The lowest detection limit for Al(3+) is 3.0 × 10(-7) M and 1.0 × 10(-7) M in EtOH and HEPES buffer respectively.
一种高效的荧光 Al(3+)受体，N-(2-羟基-1-萘基)-N'-(2-(2-羟基-1-萘基)氨基乙基)-乙烷-1,2-二胺（L），通过 2-羟基萘甲醛和二乙烯三胺之间的缩合反应合成。L 在乙醇（EtOH）和 pH 值为 7.4 的 HEPES 缓冲液中对 Al(3+)具有高选择性和亲和力，使其适合用荧光显微镜检测细胞内 Al(3+)。金属离子，如 Li(+)、Na(+)、K(+)、Mg(2+)、Ca(2+)、Mn(2+)、Co(2+)、Ni(2+)、Cu(2+)、Zn(2+)、Ag(+)、Cd(2+)、Hg(2+)和 Pb(2+)，不干扰检测。在 EtOH 和 HEPES 缓冲液中，Al(3+)的最低检测限分别为 3.0×10(-7) M 和 1.0×10(-7) M。
