人中性粒细胞前胶原酶的部分氨基酸序列

Partial amino acid sequence of human PMN leukocyte procollagenase.

作者信息

Knäuper V, Krämer S, Reinke H, Tschesche H

机构信息

University of Bielefeld, Faculty of Chemistry, Department of Biochemistry, FRG.

出版信息

Biol Chem Hoppe Seyler. 1990 May;371 Suppl:295-304.

PMID:2169256

Abstract

Human PMN leukocyte procollagenase was purified to apparent homogeneity using ion exchange chromatography on Q-Sepharose Fast Flow, affinity chromatography on Zinc Chelate Sepharose and on Orange Sepharose and was finally purified by gelpermeation chromatography on Sephacryl S-300. It was shown by SDS-PAGE under reducing and non-reducing conditions that PMNL procollagenase consists of a single polypeptide chain with an apparent Mr 85000. Amino acid sequence information was obtained from the proenzyme and fragments by Edman degradation and showed that PMNL procollagenase is homologous to the fibroblast enzyme. Thus PMNL procollagenase is the product of an independent gene.

摘要

使用Q-Sepharose Fast Flow进行离子交换色谱、在Zinc Chelate Sepharose和Orange Sepharose上进行亲和色谱，将人中性粒细胞前胶原酶纯化至表观均一性，最后通过Sephacryl S-300凝胶渗透色谱进一步纯化。在还原和非还原条件下通过SDS-PAGE显示，中性粒细胞前胶原酶由一条表观分子量为85000的单多肽链组成。通过埃德曼降解从前酶和片段获得氨基酸序列信息，结果表明中性粒细胞前胶原酶与成纤维细胞酶同源。因此，中性粒细胞前胶原酶是一个独立基因的产物。