Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde, NSW 2109, Australia.
Proteomics. 2011 Jul;11(14):2839-50. doi: 10.1002/pmic.201100068. Epub 2011 Jun 22.
Global mean surface temperature has been predicted to increase by 1.8-4°C within this century, accompanied by an increase in the magnitude and frequency of extreme temperature events. Developing rice cultivars better adapted to non-optimal temperatures is essential to increase rice yield in the future and, hence, understanding the molecular response of rice to temperature stress is necessary. In this study, we investigated the proteomic responses of leaves of 24-day-old rice seedlings to sudden temperature changes. Rice seedlings grown at 28/20°C (day/night) were subjected to 3-day exposure to 12/5°C or 20/12°C (day/night) for low-temperature stress, and 36/28°C or 44/36°C (day/night) for high-temperature stress, followed by quantitative label-free shotgun proteomic analysis on biological triplicates of each treatment. Out of over 1100 proteins identified in one or more temperature treatments, more than 400 were found to be responsive to temperature stress. Of these, 43, 126 and 47 proteins were exclusively found at 12/5, 20/12 and 44/36°C (day/night), respectively. Our results showed that a greater change occurs in the rice leaf proteome at 20/12°C (day/night) in comparison to other non-optimal temperature regimes. In addition, our study identified more than 20 novel stress-response proteins.
预计本世纪内全球平均地表温度将升高 1.8-4°C，同时极端温度事件的强度和频率也将增加。培育更好地适应非最适温度的水稻品种对于未来提高水稻产量至关重要，因此，了解水稻对温度胁迫的分子响应是必要的。在这项研究中，我们研究了 24 天大的水稻幼苗叶片对突然温度变化的蛋白质组响应。在 28/20°C（白天/夜晚）下生长的水稻幼苗，在 3 天内暴露于 12/5°C 或 20/12°C（白天/夜晚）下进行低温胁迫，以及在 36/28°C 或 44/36°C（白天/夜晚）下进行高温胁迫，然后对每个处理的三个生物学重复进行定量无标签shotgun 蛋白质组学分析。在一个或多个温度处理中鉴定出的超过 1100 种蛋白质中，有 400 多种对温度胁迫有反应。其中，43、126 和 47 种蛋白质分别仅在 12/5°C、20/12°C 和 44/36°C（白天/夜晚）下发现。我们的结果表明，与其他非最适温度条件相比，20/12°C（白天/夜晚）下水稻叶片蛋白质组发生了更大的变化。此外，我们的研究还鉴定了 20 多种新的应激响应蛋白。
