Department of Chemistry, University of Montreal, PO Box 6128, Succursale Centre-ville, Montréal, Québec H3C 3J7, Canada.
Environ Pollut. 2011 Oct;159(10):2630-6. doi: 10.1016/j.envpol.2011.05.028. Epub 2011 Jun 22.
In situ measurements provide data that are the highly representative of the natural environment. In this paper, laboratory-determined biomarkers of Cd stress that were previously identified for the green alga Chlamydomonas reinhardtii, were tested in two French rivers: a contaminated site on the Riou Mort River and an "uncontaminated" reference site on the Lot River. Transcript abundance levels were determined by real time qPCR for biomarkers thought to be Cd sensitive. Transcript levels were significantly higher (>5 fold) for organisms exposed to the contaminated site as compared to those exposed at the uncontaminated site. Biomarker mRNA levels were best correlated to free Cd (Cd(2+)) rather than intracellular Cd, suggesting that they may be useful indicators of in situ stress. The paper shows that biomarker expression levels increased with time, were sensitive to metal levels and metal speciation and were higher in the "contaminated" as opposed to the "reference" site.
原位测量提供了高度代表自然环境的数据。本文中，先前为绿藻莱茵衣藻确定的用于 Cd 胁迫的实验室生物标志物，在法国的两条河流中进行了测试：一条是 Riou Mort 河的污染地点，另一条是 Lot 河的“未污染”对照地点。通过实时 qPCR 确定了被认为对 Cd 敏感的生物标志物的转录丰度水平。与暴露在未污染地点的生物相比，暴露在污染地点的生物的转录水平显著更高（>5 倍）。生物标志物 mRNA 水平与游离 Cd（Cd(2+)）而非细胞内 Cd 相关性更好，表明它们可能是原位胁迫的有用指标。本文表明，生物标志物表达水平随时间增加，对金属水平和金属形态敏感，并且在“污染”地点高于“对照”地点。
