Department of Dermatology, Jichi Medical University, Tochigi, Japan.
Eur J Dermatol. 2011 Jul-Aug;21(4):534-8. doi: 10.1684/ejd.2011.1392.
Dermatofibroma (DF) and dermatofibrosarcoma protuberans (DFSP) are benign or intermediate malignant fibrotic dermal tumors. The contribution of transforming growth factor β (TGF-β) in the progression of sclerosis in fibrotic diseases has been implicated. To clarify the involvement of TGF-β signaling in the pathogenesis of DF or DFSP, we investigated the expression of thrombospondin-1 (TSP-1), which mediates TGF-β1 activation, on those fibrotic tumors. In the present study, we examined the expression of TSP-1 in DF and DFSP using immunohistochemical analysis and immunoblotting. In immunohistochemical staining, the expression of TSP-1 was detected weakly on epidermis and epidermal appendages, and hardly in fibroblasts in normal skin sections. The expression levels of TSP-1 were elevated in DFSP cells in comparison to normal dermal sections or DF cells. On the other hand, there was no significant difference in the protein levels in vitro of TSP-1 among normal fibroblasts, DF cells, and DFSP cells. Although the contribution of TGF-β signaling to DF or DFSP is still unknown, the expression patterns of TSP-1 in DF cells and DFSP cells may be helpful in differential diagnosis of these tumors.
隆突性皮肤纤维肉瘤（DFSP）和皮肤纤维瘤（DF）是良性或中间恶性纤维性真皮肿瘤。转化生长因子β（TGF-β）在纤维化疾病的硬化进展中的作用已被涉及。为了阐明 TGF-β信号在 DF 或 DFSP 发病机制中的作用，我们研究了介导 TGF-β1 激活的血小板反应蛋白-1（TSP-1）在这些纤维瘤中的表达。在本研究中，我们使用免疫组织化学分析和免疫印迹法检查了 TSP-1 在 DF 和 DFSP 中的表达。在免疫组织化学染色中，TSP-1 在正常皮肤切片的表皮和表皮附属物上表达较弱，而在成纤维细胞中几乎不表达。与正常真皮切片或 DF 细胞相比，DFSP 细胞中 TSP-1 的表达水平升高。另一方面，在体外，TSP-1 的蛋白水平在正常成纤维细胞、DF 细胞和 DFSP 细胞之间没有显著差异。尽管 TGF-β信号对 DF 或 DFSP 的作用仍不清楚，但 DF 细胞和 DFSP 细胞中 TSP-1 的表达模式可能有助于这些肿瘤的鉴别诊断。
