在Ha-ras癌基因转化的NIH/3T3细胞的扁平回复突变体R1中凝溶胶蛋白和α-肌动蛋白表达升高。
Elevated gelsolin and alpha-actin expression in a flat revertant R1 of Ha-ras oncogene-transformed NIH/3T3 cells.
作者信息
Müllauer L, Fujita H, Suzuki H, Katabami M, Hitomi Y, Ogiso Y, Kuzumaki N
机构信息
Laboratory of Molecular Genetics, Cancer Institute, Hokkaido University School of Medicine, Sapporo, Japan.
出版信息
Biochem Biophys Res Commun. 1990 Sep 14;171(2):852-9. doi: 10.1016/0006-291x(90)91224-g.
Expressions of gelsolin and alpha-actin have been investigated in a revertant cell line R1 and compared with the parental human activated Ha-ras oncogene-transformed NIH/3T3 (EJ-NIH/3T3), untransformed NIH/3T3 and partially revertant R2 cells. Gelsolin mRNA expression was strongest in R1 cells, intermediate in R2 and NIH/3T3 cells, and low in EJ-NIH/3T3 cells. Southern blot analysis gave neither signs of gross rearrangements nor amplification of the gelsolin gene. alpha-actin mRNA expression was restored in R1 cells to the level of NIH/3T3 cells. In R2 and EJ-NIH/3T3 cell lines, no alpha-actin transcript was detected. High gelsolin expression and restoration of alpha-actin expression may be associated with the acquirement of flat morphology and ordered cell growth pattern, which imply loss of tumorigenicity of R1 cells.
已在回复细胞系R1中研究了凝溶胶蛋白和α-肌动蛋白的表达，并与亲代人活化的Ha-ras癌基因转化的NIH/3T3（EJ-NIH/3T3）、未转化的NIH/3T3和部分回复的R2细胞进行了比较。凝溶胶蛋白mRNA表达在R1细胞中最强，在R2和NIH/3T3细胞中居中，而在EJ-NIH/3T3细胞中较低。Southern印迹分析未显示凝溶胶蛋白基因有明显重排或扩增迹象。α-肌动蛋白mRNA表达在R1细胞中恢复到NIH/3T3细胞的水平。在R2和EJ-NIH/3T3细胞系中，未检测到α-肌动蛋白转录本。高凝溶胶蛋白表达和α-肌动蛋白表达的恢复可能与扁平形态的获得和有序的细胞生长模式有关，这意味着R1细胞失去了致瘤性。
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