Matsushima R, Yoshizawa S, Watanabe M F, Harada K, Furusawa M, Carmichael W W, Fujiki H
Cancer Prevention Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1990 Sep 14;171(2):867-74. doi: 10.1016/0006-291x(90)91226-i.
Three microcystins, YR, LR and RR and nodularin, all of which are hepatotoxic compounds, inhibited dose-dependently the activity of protein phosphatase 2A in and the specific [3H]okadaic acid binding to a cytosolic fraction of mouse skin, as strongly as okadaic acid. However, microcytins and nodularin did not induce any effects on mouse skin or primary human fibroblasts. Microinjection of microcystin YR into primary human fibroblasts induced morphological changes which were induced by incubation with okadaic acid. Microcystins and nodularin penetrate into the epithelial cells of mouse skin and human fibroblasts with difficulty, which reflects tissue specificity of the compounds.
三种微囊藻毒素，即YR、LR和RR以及节球藻毒素，它们均为肝毒性化合物，与冈田酸一样，均能剂量依赖性地抑制小鼠皮肤胞质部分中蛋白磷酸酶2A的活性以及与特异性[3H]冈田酸的结合。然而，微囊藻毒素和节球藻毒素对小鼠皮肤或原代人成纤维细胞未产生任何影响。将微囊藻毒素YR显微注射到原代人成纤维细胞中会诱导出与用冈田酸孵育所诱导的形态变化。微囊藻毒素和节球藻毒素难以穿透小鼠皮肤上皮细胞和人成纤维细胞，这反映了这些化合物的组织特异性。
