Laboratory of Stem Cell and Bioevaluation, World Class University Biomodulation Program, Seoul National University, Seoul, Republic of Korea.
Biol Reprod. 2011 Oct;85(4):744-54. doi: 10.1095/biolreprod.110.084137. Epub 2011 Jun 22.
Primary follicles retrieved from B6CBAF1 prepubertal mice were cultured in a stepwise manner in an alpha-minimum essential medium-based medium to generate viable embryos and embryonic stem cell (ESC)-like cells. A significant increase in follicle growth and oocyte maturation accompanied by increased secretion of 17beta-estradiol and progesterone was achieved by exposing primary follicles to 100 or 200 mIU of follicle-stimulating hormone (FSH) during culture. More oocytes developed into blastocysts following in vitro fertilization (IVF) or parthenogenetic activation after culture with 200 mIU of FSH during the entire culture period than with 100 mIU. Eleven ESC-like cell lines, consisting of four heterozygotic and seven homozygotic phenotypes, were established from 25 trials of primary follicle culture combined with IVF or parthenogenetic activation. In conclusion, primary follicles can potentially yield developmentally competent oocytes, which produce viable embryos and ESC-like cell lines following in vitro manipulation. We suggest a method to utilize immature follicles, which are most abundant in ovaries, to improve reproductive efficiency and for use in regenerative medicine.
从 B6CBAF1 青春期前小鼠中取出的初级卵泡在逐步的 α-最小必需培养基中培养，以产生有活力的胚胎和胚胎干细胞 (ESC) 样细胞。通过在培养过程中向初级卵泡暴露 100 或 200 mIU 的卵泡刺激素 (FSH)，可以显著增加卵泡生长和卵母细胞成熟，并伴随着 17β-雌二醇和孕酮分泌的增加。在整个培养期间用 200 mIU 的 FSH 培养后，与用 100 mIU 的 FSH 相比，更多的卵母细胞在体外受精 (IVF) 或孤雌激活后发育成胚泡。从 25 次初级卵泡培养与 IVF 或孤雌激活相结合的试验中，建立了 11 个 ESC 样细胞系，包括四个杂合和七个纯合表型。总之，初级卵泡有可能产生具有发育能力的卵母细胞，这些卵母细胞经过体外操作后可以产生有活力的胚胎和 ESC 样细胞系。我们建议利用卵巢中最丰富的未成熟卵泡来提高生殖效率并用于再生医学的方法。
