Toyonaga Mari, Kaihara Aya, Tsutsui Toshihiko
Department of Reproduction, Nippon Veterinary and Life Science University, Tokyo, Japan.
J Vet Med Sci. 2011 Oct;73(10):1395-8. doi: 10.1292/jvms.11-0052. Epub 2011 Jun 22.
On the assumption that animals of wild feline species died in the field, caudal epididymal sperm were cryopreserved following storage of the feline epididymides at 20°C for 0-24 hr, and their qualities were observed. Compared to the qualities at 0 hr, no significant differences were noted following 12 hr of storage at 20°C. On comparison of the qualities between caudal sperm cryopreserved after 24 hr storage at 4°C and after 12 hr at 20°C followed by 12 hr storage at 4°C, no significant differences were noted. These findings suggest that the cryopreserved sperm collected from epididymides of dead animals might be useful for artificial insemination if cryopreservation was performed within 12 hr exposure to ambient temperature.
假设野生猫科动物在野外死亡,将猫附睾在20°C下保存0 - 24小时后,对附睾尾部精子进行冷冻保存,并观察其质量。与0小时时的质量相比,在20°C下保存12小时后未发现显著差异。比较在4°C下保存24小时后冷冻保存的尾部精子与在20°C下保存12小时后再在4°C下保存12小时后冷冻保存的尾部精子的质量,未发现显著差异。这些发现表明,如果在暴露于环境温度12小时内进行冷冻保存,从死亡动物附睾中收集的冷冻保存精子可能对人工授精有用。