Biological Resource Center (BRC), Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong-gu, Daejeon 305-806, Korea.
Biotechnol Lett. 2011 Oct;33(10):2043-8. doi: 10.1007/s10529-011-0668-7. Epub 2011 Jun 23.
The electron transfer pathway of Geobacter sulfurreducens has been intensively studied because of its ability of electron transfer to extracellular electron acceptors, such as Fe(III) and on electrode. However, the absence of overexpression system of G. sulfurreducens is one of the main obstacles for studying the physiology of G. sulfurreducens. OmcZ, an outer membrane-related c-type cytochrome of G. sulfurrducens, was homologously overexpressed via genomic integration. Quantitative RT-PCR analysis showed that the omcZ transcript in the knock-in strain was sixfold more abundant than in the wild type. Notably, omcZ expression appears to downregulate the expression level of OmcS, another outer membrane-related c-type cytochrome of G. sulfurreducens, based on the comparisons of total protein and transcript levels. This is the first report of the successful genetic overexpression system for studying functional genomics of G. sulfurreducens.
由于能够将电子传递给胞外电子受体（如 Fe(III) 和电极），因此人们对 Geobacter sulfurreducens 的电子转移途径进行了深入研究。然而，G. sulfurreducens 缺乏过表达系统是研究其生理学的主要障碍之一。OmcZ 是 G. sulfurreducens 的一种外膜相关 c 型细胞色素，通过基因组整合进行同源过表达。定量 RT-PCR 分析表明，敲入菌株中的 omcZ 转录本比野生型丰富六倍。值得注意的是，根据总蛋白和转录本水平的比较，omcZ 的表达似乎下调了 G. sulfurreducens 的另一种外膜相关 c 型细胞色素 OmcS 的表达水平。这是首次成功建立用于研究 G. sulfurreducens 功能基因组学的遗传过表达系统的报道。
