Ambar I, Kloog Y, Sokolovsky M
Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
Biochemistry. 1990 Jul 10;29(27):6415-8. doi: 10.1021/bi00479a012.
Affinity-labeling experiments with 125I-endothelin derivatives using bifunctional cross-linking reagents were carried out in an attempt to identify the polypeptide component(s) of the endothelin/sarafotoxin receptors in rat brain tissues. In rat cerebellum, cortex, and caudate putamen, endothelin 1 specifically labeled a major component with a molecular mass of around 53,000. In the same tissues endothelin 3 specifically labeled, in addition to the 53,000 band, a band of molecular mass of 38,000. This result clearly indicates that in the brain the endothelin binding site resides within a polypeptide of apparent Mr = 53,000. The possible presence of receptor subtypes is discussed with reference also to the reported identification of endothelin receptors in chick cardiac membrane and in rat mesangial cells.
使用双功能交联剂对125I-内皮素衍生物进行亲和标记实验，旨在鉴定大鼠脑组织中内皮素/铃蟾毒素受体的多肽成分。在大鼠小脑、皮质和尾状壳核中，内皮素1特异性标记了一个分子量约为53,000的主要成分。在相同组织中，内皮素3除了标记53,000条带外，还特异性标记了一条分子量为38,000的条带。该结果清楚地表明，在大脑中，内皮素结合位点位于表观分子量为53,000的多肽内。还参考了在鸡心肌膜和大鼠系膜细胞中报道的内皮素受体鉴定结果，讨论了受体亚型可能存在的情况。
