芒属（禾本科）微卫星标记的开发及其在其他相关物种中的交叉扩增。

Development of microsatellite markers for Miscanthus sinensis (Poaceae) and cross-amplification in other related species.

机构信息

State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.

出版信息

Am J Bot. 2011 Jul;98(7):e195-7. doi: 10.3732/ajb.1100040. Epub 2011 Jun 23.

DOI:10.3732/ajb.1100040

PMID:21700800

Abstract

PREMISE OF THE STUDY

We developed microsatellite loci for the biomass crop Miscanthus sinensis to investigate genetic diversity and population structure of M. sinensis and its closely related species.

METHODS AND RESULTS

Fourteen microsatellite loci were identified from an enriched genomic library of M. sinensis and tested in one M. sinensis population. The number of alleles per locus ranged from 1 to 15, with a mean of 7.0. The observed and expected heterozygosities varied from 0.318 to 0.864 and from 0.424 to 0.901, respectively. Of them, 12 primers could be applied to three other species in Miscanthus (M. sacchariflorus, M. floridulus, and M. lutarioriparius).

CONCLUSIONS

These markers will be important for further analyzing population genetics and evolutionary history, as well as facilitating molecular breeding of Miscanthus sinensis and its related species.

摘要

研究前提

我们开发了生物质作物芒属的微卫星标记，以研究芒属及其近缘种的遗传多样性和种群结构。

方法和结果

从芒属的富集基因组文库中鉴定出 14 个微卫星位点，并在一个芒属种群中进行了测试。每个位点的等位基因数从 1 到 15 不等，平均值为 7.0。观察到的和预期的杂合度分别从 0.318 到 0.864 和从 0.424 到 0.901 不等。其中，12 个引物可应用于芒属的另外三个种（荻、短芒荻和五节芒）。