用于生物能源草芒属（禾本科）的微卫星位点的分离和特征。
Isolation and characterization of microsatellite loci for a bioenergy grass, Miscanthus sacchariflorus (Poaceae).
机构信息
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, People's Republic of China.
出版信息
Appl Plant Sci. 2013 Jan 2;1(1). doi: 10.3732/apps.1200210. eCollection 2013 Jan.
PREMISE OF THE STUDY
Microsatellite loci were developed for the biomass C4 grass, Miscanthus sacchariflorus, and proved to be suitable markers for population genetic studies and germplasm management of this species. •
METHODS AND RESULTS
Twenty-three polymorphic microsatellite loci were identified from an enriched genomic library of M. sacchariflorus. The polymorphism was assessed in 50 individuals from two populations in China. The number of alleles per locus varied from two to 18, with a mean of 8.13. The observed and expected heterozygosities ranged from 0.2 to 1.0 and from 0.198 to 0.898, respectively. •
CONCLUSIONS
These new markers will be useful for further investigation of genetic diversity and population genetic structure as well as molecular breeding of Miscanthus species.
研究前提
开发了用于生物质 C4 草芒属植物的微卫星位点，这些位点被证明是该物种种群遗传研究和种质管理的合适标记。
方法和结果
从芒属植物的富集基因组文库中鉴定出 23 个多态性微卫星位点。在中国的两个种群的 50 个个体中评估了多态性。每个位点的等位基因数从 2 到 18 不等，平均值为 8.13。观察到的和预期的杂合度分别在 0.2 到 1.0 和 0.198 到 0.898 之间。
结论
这些新标记将有助于进一步研究芒属物种的遗传多样性和种群遗传结构，以及分子育种。
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