分离鉴定二型花柱植物长距球兰的微卫星标记。

Isolation and characterization of microsatellite markers in distylous Palicourea padifolia (Rubiaceae).

机构信息

Departamento de Biología Evolutiva, Instituto de Ecología, AC, Carretera Antigua a Coatepec No. 351, El Haya, Xalapa, Veracruz 91070, México.

出版信息

Am J Bot. 2011 Jul;98(7):e164-6. doi: 10.3732/ajb.1100042. Epub 2011 Jun 23.

DOI:10.3732/ajb.1100042

PMID:21700802

Abstract

PREMISE OF THE STUDY

Eighteen microsatellite loci of distylous Palicourea padifolia were isolated and characterized for population genetics studies.

METHODS AND RESULTS

Following a microsatellite enrichment protocol, 18 primer pairs amplified successfully, and the polymorphism of the loci was initially evaluated in 15 individuals from three populations in Mexico. Seven loci were polymorphic, and their variability was further assessed in 60 individuals from three populations. The total number of alleles per locus, combining samples from all populations, ranged from 7 to 12. Nei's genetic diversity average across loci was 0.681, 0.714, and 0.703 for El Riscal, Montebello, and Ruiz Cortínez, respectively. Palicourea padifolia individuals had one to four alleles, confirming the polyploidy of this species.

CONCLUSIONS

These markers will facilitate genetic diversity studies of P. padifolia across its distribution range and facilitate investigations on the evolution of its breeding system.

摘要

研究前提

从雌雄同体的 Palicourea padifolia 中分离和鉴定了 18 个微卫星位点，用于群体遗传学研究。

方法与结果

采用微卫星富集方案，成功扩增了 18 对引物，初步评估了来自墨西哥三个种群的 15 个个体中这些位点的多态性。七个位点具有多态性，进一步评估了来自三个种群的 60 个个体中这些位点的变异性。每个位点的等位基因总数，将所有种群的样本结合起来，范围从 7 到 12。Nei 的遗传多样性平均值分别为 El Riscal、Montebello 和 Ruiz Cortínez 的 0.681、0.714 和 0.703。Palicourea padifolia 个体具有一到四个等位基因，证实了该物种的多倍性。