中国濒危特有种伯乐树（无患子科）微卫星标记的分离与特征分析。
Isolation and characterization of microsatellite markers for Dipteronia dyerana (Sapindaceae), an endangered endemic species in China.
机构信息
Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, Zhejiang University, Hangzhou, China.
出版信息
Am J Bot. 2011 Oct;98(10):e271-3. doi: 10.3732/ajb.1100185.
PREMISE OF THE STUDY
Microsatellite markers were developed for Dipteronia dyerana, an endangered endemic species in China, to study the population genetics of this species.
METHODS AND RESULTS
Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 12 microsatellite markers that were successfully amplified showed polymorphism when tested on 31 individuals from two populations in the counties of Wenshan and Mengzi. Overall, the number of alleles per locus ranged between seven and 25, with an average of 12.3. Nine of these markers were able to be amplified in D. sinensis.
CONCLUSIONS
These microsatellite markers should facilitate further studies on the population genetics and the reproductive ecology of Dipteronia.
研究前提
为中国特有的濒危物种滇桐（Dipteronia dyerana）开发了微卫星标记，以研究该物种的种群遗传学。
方法和结果
使用基于重复序列的快速 AFLP 片段分离（FIASCO）方案，从文山和蒙自两县的两个种群的 31 个个体中成功扩增的 12 个微卫星标记显示出多态性。总体而言，每个位点的等位基因数在 7 到 25 之间，平均为 12.3。其中 9 个标记可以在滇桐中扩增。
结论
这些微卫星标记应有助于进一步研究滇桐的种群遗传学和生殖生态学。
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