清道夫受体配体的鉴定。

Identification of scavenger receptor ligands.

作者信息

Neyen Claudine, Pluddemann Annette, Gordon Siamon

机构信息

Department of Biochemistry, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.

出版信息

Methods Mol Biol. 2011;748:35-50. doi: 10.1007/978-1-61779-139-0_3.

DOI:10.1007/978-1-61779-139-0_3

PMID:21701965

Abstract

Scavenger receptors (SRs) are structurally diverse but functionally related innate immune receptors involved in defence and clearance mechanisms. Their broad specificity relies on evolutionarily conserved pattern recognition domains which interact with a variety of microbial, apoptotic and modified self ligands. Studies of immune functions of SR-expressing cells require the identification of interaction partners for SRs. We have developed an ELISA-based method which allows for large-scale high-throughput screening of complex mixtures. The assay successfully identified bacterial and plasma ligands for macrophage scavenger receptor A and can be adapted to screen for novel exogenous or endogenous ligands for any immune receptor of interest.

摘要

清道夫受体（SRs）在结构上具有多样性，但在功能上是相关的天然免疫受体，参与防御和清除机制。它们广泛的特异性依赖于进化上保守的模式识别结构域，这些结构域与多种微生物、凋亡和修饰的自身配体相互作用。对表达SR的细胞的免疫功能研究需要鉴定SR的相互作用伙伴。我们开发了一种基于酶联免疫吸附测定（ELISA）的方法，该方法可用于对复杂混合物进行大规模高通量筛选。该检测方法成功鉴定出巨噬细胞清道夫受体A的细菌和血浆配体，并且可以进行调整以筛选任何感兴趣的免疫受体的新型外源性或内源性配体。