通过光漂白后荧光恢复测量免疫受体的流动性。

Measuring immune receptor mobility by fluorescence recovery after photobleaching.

作者信息

Silver Kristen, Harrison Rene E

机构信息

Departments of Biological Sciences and Cell and Systems Biology, University of Toronto-Scarborough, Toronto, Ontario, Canada.

出版信息

Methods Mol Biol. 2011;748:155-67. doi: 10.1007/978-1-61779-139-0_11.

DOI:10.1007/978-1-61779-139-0_11

PMID:21701973

Abstract

The coordinated effort of cells in the immune system relies heavily on surface receptor interactions. Immune receptor mobility provides vital information on the function and responses of immune cells, and these measurements shed light on their interactions with other membrane, cytosolic, and extracellular matrix proteins. These measurements can be obtained using the fluorescence recovery after photobleaching (FRAP) technique in living cells. We describe here general approaches for FRAP using green fluorescent protein fusion proteins.

摘要

免疫系统中细胞的协同作用在很大程度上依赖于表面受体的相互作用。免疫受体的流动性提供了关于免疫细胞功能和反应的重要信息，这些测量结果揭示了它们与其他膜蛋白、胞质蛋白和细胞外基质蛋白的相互作用。这些测量可以在活细胞中使用光漂白后荧光恢复（FRAP）技术来获得。我们在此描述使用绿色荧光蛋白融合蛋白进行FRAP的一般方法。