Lai Yan, Xu Bo, He Li, Lin Ming, Cao Lei, Mou Shaoliang, Wu Yang, He Shuilin
School of Life Sciences, Fujian Agriculture and Forestry University, 350002 Fuzhou, China.
Indian J Exp Biol. 2011 Jun;49(6):429-37.
In the present paper, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) was used to examine and identify differentially expressed genes in Capsicum annuum exposed to UV-B irradiation. Around 4000 transcript derived fragments (TDFs) were visualized and in total 183 TDFs were isolated, sequenced and analyzed by Blast 2 go. Among these TDFs, 84 of them showed homology to known genes. There were 43 TDFs showing up-regulated expression, 24 TDFs showing down-regulated expression and 29 TDFs showing both up-regulated and down-regulated expression, respectively. Some of these TDFs were found to be in response/related to UV-B stress, including carbonic anhydrase, calcium-dependent protein, thionin-like protein, bzip protein and so on. In particular, chlorophyll a/b binding protein (Capcab) responding to UV-B stress was cloned. It was concluded that Capcab could play a protective role in plant anti-UV-B and maintaining photosynthetic rate under UV-B stress.
在本论文中，互补DNA扩增片段长度多态性（cDNA-AFLP）被用于检测和鉴定暴露于UV-B辐射下的辣椒中差异表达的基因。大约4000个转录本衍生片段（TDFs）被可视化，总共分离出183个TDFs，进行测序并通过Blast 2 go分析。在这些TDFs中，84个与已知基因具有同源性。分别有43个TDFs表现为上调表达，24个TDFs表现为下调表达，29个TDFs表现为既有上调表达又有下调表达。发现其中一些TDFs对UV-B胁迫有响应/相关，包括碳酸酐酶、钙依赖性蛋白、类硫堇蛋白、bZIP蛋白等。特别地，克隆了响应UV-B胁迫的叶绿素a/b结合蛋白（Capcab）。得出的结论是，Capcab在植物抗UV-B以及在UV-B胁迫下维持光合速率方面可能发挥保护作用。
