Hannan Abdul, Humayun Tabish, Hussain Muhammad Barkaat, Yasir Muhammad, Sikandar Sumayya
Department of Microbiology, University of Health Sciences, Lahore, Pakistan.
J Ayub Med Coll Abbottabad. 2010 Apr-Jun;22(2):160-3.
Cholera is a major public health problem in developing countries of the world. Bacterial resistance, lack of surveillance data and proper microbiological facilities are major problems regarding diagnosis of cholera. The spread of microbial drug resistance is a global public health challenge that results in increased illness and death rate. Newer antimicrobials or agents are urgently required to overcome this problem. This work was therefore done to investigate the antimicrobial potential of onion against thirty-three clinical isolates of Vibrio cholerae.
The extract was prepared by reflux extraction method. Antibacterial screening of clinical isolates of V. cholerae was done by agar well diffusion method. Agar dilution method was used to assess the Minimum Inhibitory Concentration (MIC).
All tested strains of V. cholerae were sensitive to onion (Allium cepa) extracts of two types (purple and yellow). Purple type of extract had MIC range of 19.2-21.6 mg/ml. The extract of yellow type onion had an MIC range of 66-68.4 mg/ml.
The results indicated that onion (Allium cepa) has an inhibitory effect on V. cholerae. Keeping in view the anti-bacterial activity of this compound can be exploited as a therapeutic agent in an animal model. This finding is a positive point for further investigation of this herb of traditional medicine.
霍乱是世界上发展中国家的一个主要公共卫生问题。细菌耐药性、缺乏监测数据以及适当的微生物学设施是霍乱诊断方面的主要问题。微生物耐药性的传播是一项全球公共卫生挑战，会导致疾病和死亡率上升。迫切需要更新型的抗菌药物或制剂来克服这一问题。因此开展这项工作以研究洋葱对33株霍乱弧菌临床分离株的抗菌潜力。
采用回流提取法制备提取物。通过琼脂孔扩散法对霍乱弧菌临床分离株进行抗菌筛选。采用琼脂稀释法评估最低抑菌浓度（MIC）。
所有测试的霍乱弧菌菌株均对两种类型（紫色和黄色）的洋葱（葱属）提取物敏感。紫色类型提取物的MIC范围为19.2 - 21.6毫克/毫升。黄色类型洋葱提取物的MIC范围为66 - 68.4毫克/毫升。
结果表明洋葱（葱属）对霍乱弧菌有抑制作用。鉴于该化合物的抗菌活性，可在动物模型中作为治疗剂加以利用。这一发现为进一步研究这种传统草药提供了一个积极的切入点。
